John T M, George J C, Moran E T
Cytobios. 1987;49(198-199):197-210.
Hatching muscle of turkey embryos from eggs previously dipped in an antibiotic solution with or without glucose, was studied at three developmental stages, 'knocking', 'pipping' and 'post-hatch'. In both treated and untreated groups, occurrence of relaxed muscle fibres was more characteristic of pre-hatch than post-hatch stages. Glycogen decreased toward post-hatch in all groups while lactate increased in untreated and antibiotic groups. In the antibiotic group, glycogen was lower (all stages) and lactate higher (pre-hatch stages) than in the untreated group. In the glucose-added group, there was no reduction in glycogen, but glycolysis seemed to be further enhanced (as indicated by higher lactate) in pre-hatch stages. Thus, providing eggs with glucose may help maintain higher muscle glycogen level even during active glycolysis. The free fatty acid (FFA) level in the untreated group progressively increased from knocking to post-hatch, indicating mobilization of fat as fuel. The antibiotic group had a higher FFA level than the untreated group during knocking and pipping. In the glucose-added group, FFA at knocking was even higher than that at the corresponding stage in the antibiotic group.
研究了预先浸泡在含或不含葡萄糖的抗生素溶液中的火鸡胚胎蛋在三个发育阶段（“敲壳”、“啄壳”和“出壳后”）的孵化肌肉情况。在处理组和未处理组中，松弛肌纤维的出现都是孵化前阶段比孵化后阶段更具特征。所有组的糖原在出壳后都减少，而未处理组和抗生素处理组的乳酸增加。在抗生素处理组中，糖原（在所有阶段）低于未处理组，乳酸（在孵化前阶段）高于未处理组。在添加葡萄糖的组中，糖原没有减少，但在孵化前阶段糖酵解似乎进一步增强（以更高的乳酸水平为指标）。因此，给蛋提供葡萄糖甚至在活跃糖酵解期间也可能有助于维持更高的肌肉糖原水平。未处理组的游离脂肪酸（FFA）水平从敲壳到出壳后逐渐增加，表明脂肪作为燃料被动员。在敲壳和啄壳期间，抗生素处理组的FFA水平高于未处理组。在添加葡萄糖的组中，敲壳时的FFA甚至高于抗生素处理组相应阶段的水平。
