John T M, George J C, Moran E T
Department of Zoology, University of Guelph, Ontario, Canada.
Poult Sci. 1988 Mar;67(3):463-9. doi: 10.3382/ps.0670463.
Small type turkey eggs were pressure differential dipped in an antibiotic solution (spectinomycin 3,000 ppm) with or without glucose (10%) and incubated. Pectoral muscle and liver samples taken from the embryos at the knocking, pipping, and posthatch stages of development were studied. Muscle glycogen decreased between pipping and posthatch. Liver glycogen decreased progressively between knocking and posthatch. Whereas liver free fatty acids decreased as the embryo completed the hatching process, muscle levels were less subject to change. Providing the embryo with antibiotic plus glucose improved overall glycogen in both tissues and reduced muscle lactate. Results from treatment with antibiotic alone resembled those in the first treatment with regard to liver glycogen and muscle lactate but to a lesser degree. Results support the hypothesis of an extensive muscle involvement in the hatching process and need for glucose through the perihatch period.
将小型火鸡种蛋在有或没有葡萄糖（10%）的抗生素溶液（壮观霉素3000 ppm）中进行压差浸泡，然后孵化。对在发育的啄壳、破壳和出壳后阶段从胚胎采集的胸肌和肝脏样本进行了研究。胸肌糖原在破壳和出壳后之间减少。肝脏糖原在啄壳和出壳后之间逐渐减少。随着胚胎完成孵化过程，肝脏游离脂肪酸减少，而肌肉水平变化较小。给胚胎提供抗生素加葡萄糖可改善两个组织中的总体糖原含量，并降低肌肉乳酸含量。单独使用抗生素治疗的结果在肝脏糖原和肌肉乳酸方面与首次治疗相似，但程度较轻。结果支持了肌肉在孵化过程中广泛参与以及在孵化期需要葡萄糖的假设。
