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RovM和CsrA对……中的脲酶表达起负调控作用。 （你提供的原文不完整，这里补充了“对……中的”以使句子更通顺，但严格按照要求，应根据完整原文准确翻译。）

RovM and CsrA Negatively Regulate Urease Expression in .

作者信息

Dai Qingyun, Xu Lei, Xiao Lu, Zhu Kaixiang, Song Yunhong, Li Changfu, Zhu Lingfang, Shen Xihui, Wang Yao

机构信息

State Key Laboratory of Crop Stress Biology for Arid Areas and College of Life Sciences, Northwest A&F University, Yangling, China.

College of Life Sciences, Northwest A&F University, Yangling, China.

出版信息

Front Microbiol. 2018 Feb 27;9:348. doi: 10.3389/fmicb.2018.00348. eCollection 2018.

DOI:10.3389/fmicb.2018.00348
PMID:29535702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5835112/
Abstract

Urease acts as an important acid resistance system and virulence factor that is widespread among microorganisms. RovM is a global regulator that regulates a series of genes and pathways including acid survival systems in the enteric bacterium . However, whether RovM regulates the urease activity in was still unknown. In this study, by using qualitative and quantitative urease assays, we show that the urease expression responds to nutrient conditions and the RovM protein represses urease expression by binding to its promoter. A previously reported positive regulator OmpR activates urease activity but RovM plays a dominant role in different nutrient conditions. In addition, carbon storage regulator system A (CsrA), the upstream regulator of RovM, dramatically down-regulates urease activity possibly by its binding to the Shine-Dalgarno (SD) sequence of the mRNA encoding the urease. In conclusion, this study demonstrates that urease activity is strictly controlled by nutrient conditions and is down-regulated by the CsrA-RovM pathway.

摘要

脲酶作为一种重要的耐酸系统和毒力因子，广泛存在于微生物中。RovM是一种全局调节因子，可调节包括肠道细菌中的酸存活系统在内的一系列基因和途径。然而，RovM是否调节脲酶活性仍不清楚。在本研究中，通过定性和定量脲酶测定，我们表明脲酶表达对营养条件有反应，并且RovM蛋白通过与其启动子结合来抑制脲酶表达。先前报道的正调节因子OmpR激活脲酶活性，但RovM在不同营养条件下起主导作用。此外，RovM的上游调节因子碳储存调节系统A（CsrA）可能通过与编码脲酶的mRNA的Shine-Dalgarno（SD）序列结合而显著下调脲酶活性。总之，本研究表明脲酶活性受到营养条件的严格控制，并通过CsrA-RovM途径下调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/509811ad6b4b/fmicb-09-00348-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/0971c24a0411/fmicb-09-00348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/387cb96e829f/fmicb-09-00348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/e703bf6da37f/fmicb-09-00348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/c2c7086660cd/fmicb-09-00348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/8b54942284c1/fmicb-09-00348-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/bef9d72067ff/fmicb-09-00348-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/509811ad6b4b/fmicb-09-00348-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/0971c24a0411/fmicb-09-00348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/387cb96e829f/fmicb-09-00348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/e703bf6da37f/fmicb-09-00348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/c2c7086660cd/fmicb-09-00348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/8b54942284c1/fmicb-09-00348-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/bef9d72067ff/fmicb-09-00348-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64fd/5835112/509811ad6b4b/fmicb-09-00348-g007.jpg

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