Tatarunas Vacis, Voleisis Algirdas, Sliteris Reimondas, Kazys Rymantas, Mazeika Liudas, Lesauskaite Vaiva
Laboratory of Molecular Cardiology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu 15, 50009, Kaunas, Lithuania.
Ultrasound Institute, Kaunas University of Technology, K. Baršausko g. 59, Kaunas, Lithuania.
J Med Ultrason (2001). 2018 Oct;45(4):545-553. doi: 10.1007/s10396-018-0874-1. Epub 2018 Mar 13.
For long time, blood clot retraction was measured only by thromboelastographic or platelet contractile force measurement techniques. The purpose of the present study was development of a novel ultrasonic method based on simultaneous monitoring of variations in the ultrasound velocity and the frequency spectrum of the signal propagating in clotting blood and its application for automatic evaluation of blood clotting parameters.
Simultaneous measurement of ultrasound velocity and variations in the frequency spectrum of wideband ultrasonic signals in clotting blood samples was performed. All measurements were performed in pulse-echo mode. Standard clinical data were obtained using routine clinical laboratory methods.
The amplitudes of ultrasonic signals during native blood coagulation varied up to ten times for different frequencies. The measurement results of the start and duration of blood clot retraction differed between patient samples: different components of the blood coagulation system had significant impact on the blood clot retraction process.
Our results showed that during blood clotting, the ultrasound velocity and variations in frequency spectrum should be used simultaneously to determine the beginning and duration of blood clot retraction. Our results also showed that blood clot retraction is controlled by the activity of factor XIII.
长期以来，血凝块回缩仅通过血栓弹力图或血小板收缩力测量技术来测定。本研究的目的是开发一种基于同时监测凝血血液中传播信号的超声速度和频谱变化的新型超声方法，并将其应用于自动评估凝血参数。
对凝血血液样本中的超声速度和宽带超声信号频谱变化进行同步测量。所有测量均在脉冲回波模式下进行。使用常规临床实验室方法获取标准临床数据。
在天然血液凝固过程中，不同频率的超声信号幅度变化高达十倍。患者样本之间血凝块回缩开始和持续时间的测量结果不同：凝血系统的不同成分对血凝块回缩过程有显著影响。
我们的结果表明，在血液凝固过程中，应同时使用超声速度和频谱变化来确定血凝块回缩的开始和持续时间。我们的结果还表明，血凝块回缩受因子 XIII 活性的控制。
