College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
Bioresour Technol. 2018 Jul;259:10-17. doi: 10.1016/j.biortech.2018.03.024. Epub 2018 Mar 7.
The increasing use of engineered Ag nanoparticles (NPs) in consumer products raises great concerns about their environmental impacts, but their potential impact on anaerobic ammonium oxidation (anammox) bacteria in wastewater treatment remains unclear. In this study, the response of anammox consortia inhabiting in granules to long-term exposure of AgNPs was investigated. The gradually increased stress of AgNPs (1, 10, 50 mg L) showed no adverse effects on the nitrogen removal performance and on the relative abundance of Ca. Kuenenia inhabiting in granules. Notably, in response to the escalating burden of AgNPs, anammox consortia tended to secrete more protein but less polysaccharide, and meanwhile increase the relative abundances of Ag efflux protein SilA and regulatory protein SilR. Overall, these results suggested that the strong resistance of anammox granules to AgNPs might be mainly attributed to the defense role of extracellular polymeric substances and gene-based regulation of ion transport.
越来越多的工程化银纳米粒子（AgNPs）被应用于消费产品，这引发了人们对其环境影响的极大关注，但它们对废水处理中厌氧氨氧化（anammox）细菌的潜在影响尚不清楚。在本研究中，我们考察了长期暴露于 AgNPs 对颗粒体内 anammox 菌群的影响。AgNPs（1、10、50mg/L）的逐渐增加的胁迫并未对氮去除性能和颗粒体内 Kuenenia 的相对丰度产生不利影响。值得注意的是，为应对 AgNPs 的不断增加的负担，anammox 菌群倾向于分泌更多的蛋白质但更少的多糖，同时增加 Ag 外排蛋白 SilA 和调节蛋白 SilR 的相对丰度。总的来说，这些结果表明，anammox 颗粒对 AgNPs 的强抗性可能主要归因于胞外聚合物的防御作用和基于基因的离子转运调控。
