College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, Hangzhou Normal University, Hangzhou 310036, China.
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China.
Bioresour Technol. 2017 Oct;241:35-43. doi: 10.1016/j.biortech.2017.05.069. Epub 2017 May 15.
The increasing use of engineered nanoparticles (NPs) poses an emerging challenge to biological wastewater treatment. The long-term impact of CuNPs on anaerobic ammonium oxidation (anammox) process was firstly investigated in this study. The nitrogen removal capacity of anammox reactor was nearly deprived within 30days under the stress of 5.0mgL CuNPs and the relative abundance of anammox bacteria (Ca. Kuenenia) was decreased from 29.59% to 17.53%. Meanwhile, copper resistance genes associated with the Cus, Cop and Pco systems were enriched to eliminate excess intracellular copper. After the withdrawal of CuNPs from the influent, the nitrogen removal capacity of anammox biomass recovered completely within 70days. Overall, anammox biomass showed susceptibility, resistance and resilience to the stress of CuNPs. Therefore, the potential impacts of ENPs on anammox-based processes should be of great concern.
工程纳米粒子（NPs）的使用日益增多，对生物废水处理构成了新的挑战。本研究首次探讨了 CuNPs 对厌氧氨氧化（anammox）工艺的长期影响。在 5.0mg/L CuNPs 的压力下，anammox 反应器的脱氮能力在 30 天内几乎耗尽，anammox 细菌（Ca. Kuenenia）的相对丰度从 29.59%下降到 17.53%。同时，与 Cus、Cop 和 Pco 系统相关的铜抗性基因被富集以消除过量的细胞内铜。当 CuNPs 从进水中断流后，anammox 生物量的脱氮能力在 70 天内完全恢复。总的来说，anammox 生物量对 CuNPs 的压力表现出敏感性、抗性和弹性。因此，ENPs 对基于 anammox 的工艺的潜在影响应该引起极大的关注。
