Flügge G, Inagami T, Fuchs E
Histochemistry. 1987;86(5):479-83. doi: 10.1007/BF00500620.
ANP (atrial natriuretic peptide), a peptide found in granules of mammalian atrial cardiac myocytes, has been shown to be active in regulation of blood pressure and body water homeostasis. The existence of ANP in atrium, pituitary, adrenal gland, and kidney of the rat had been immunocytochemically demonstrated with an antibody against rat ANP (102-126). We used the same antibody in immunocytochemical studies for the detection of ANP in peripheral organs of the tree shrew (Tupaia belangeri). The antibody stained granules in myocytes of cardiac atria which indicated that it reacted with tree shrew ANP. In contrast to the rat, no immunoreactive cells were found in pituitaries and adrenal glands. However, in the kidneys distal tubules in outer medulla and cortex were labeled. Ascending limbs of distal tubules were intensely stained when either the peroxidase-antiperoxidase (PAP) or the indirect immunofluorescence method were used. Collecting ducts and convoluted distal tubules in the outer cortex showed a granular type of staining when the immunofluorescence method was used. These data indicate that ANP is present in epithelial cells of distal tubules and collecting ducts, where it may be involved in the regulation of renal salt excretion.
心房利钠肽（ANP）是一种在哺乳动物心房心肌细胞颗粒中发现的肽，已被证明在调节血压和体内水平衡方面具有活性。用抗大鼠ANP（102 - 126）抗体通过免疫细胞化学方法已证实大鼠的心房、垂体、肾上腺和肾脏中存在ANP。我们使用相同的抗体进行免疫细胞化学研究，以检测树鼩（Tupaia belangeri）外周器官中的ANP。该抗体对心房肌细胞中的颗粒进行了染色，这表明它与树鼩ANP发生了反应。与大鼠不同，在垂体和肾上腺中未发现免疫反应性细胞。然而，在外髓质和皮质的肾远曲小管中被标记。当使用过氧化物酶 - 抗过氧化物酶（PAP）法或间接免疫荧光法时，远曲小管的升支被强烈染色。当使用免疫荧光法时，外皮质中的集合管和迂曲远曲小管呈现颗粒状染色。这些数据表明ANP存在于远曲小管和集合管的上皮细胞中，在那里它可能参与肾盐排泄的调节。
