利用10.6微米的光使限制在纳米液滴中的蛋白质熔化，为变性的早期步骤提供了线索。
Melting proteins confined in nanodroplets with 10.6 μm light provides clues about early steps of denaturation.
作者信息
El-Baba Tarick J, Fuller Daniel R, Woodall Daniel W, Raab Shannon A, Conant Christopher R, Dilger Jonathan M, Toker Yoni, Williams Evan R, Russell David H, Clemmer David E
机构信息
Department of Chemistry, Indiana University, 800 Kirkwood Avenue, Bloomington, Indiana, 47401, USA.
出版信息
Chem Commun (Camb). 2018 Mar 27;54(26):3270-3273. doi: 10.1039/c7cc09829d.
Abstract
Ubiquitin confined within nanodroplets was irradiated with a variable-power CO2 laser. Mass spectrometry analysis shows evidence for a protein "melting"-like transition within droplets prior to solvent evaporation and ion formation. Ion mobility spectrometry reveals that structures associated with early steps of denaturation are trapped because of short droplet lifetimes.
摘要
用可变功率的二氧化碳激光照射限制在纳米液滴内的泛素。质谱分析表明，在溶剂蒸发和离子形成之前，液滴内存在类似蛋白质“熔化”的转变。离子迁移谱显示，由于液滴寿命短，与变性早期步骤相关的结构被捕获。
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Analyst. 2017 Jun 12;142(12):2152-2160. doi: 10.1039/c7an00362e.
2
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3
Increasing Ubiquitin Ion Resistance to Unfolding in the Gas Phase Using Chloride Adduction: Preserving More "Native-Like" Conformations Despite Collisional Activation.
Anal Chem. 2016 Jun 7;88(11):5934-40. doi: 10.1021/acs.analchem.6b00871. Epub 2016 May 10.
4
Ultrafast (1 μs) Mixing and Fast Protein Folding in Nanodrops Monitored by Mass Spectrometry.
J Am Chem Soc. 2016 Mar 16;138(10):3453-60. doi: 10.1021/jacs.5b13081. Epub 2016 Mar 2.
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J Am Soc Mass Spectrom. 2015 Sep;26(9):1433-43. doi: 10.1007/s13361-015-1191-1. Epub 2015 Jun 27.
6
Microdroplet fusion mass spectrometry for fast reaction kinetics.
Proc Natl Acad Sci U S A. 2015 Mar 31;112(13):3898-903. doi: 10.1073/pnas.1503689112. Epub 2015 Mar 16.
7
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J Am Chem Soc. 2014 Jul 23;136(29):10308-14. doi: 10.1021/ja502994b. Epub 2014 Jul 9.
8
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9
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