El-Baba Tarick J, Fuller Daniel R, Woodall Daniel W, Raab Shannon A, Conant Christopher R, Dilger Jonathan M, Toker Yoni, Williams Evan R, Russell David H, Clemmer David E
Department of Chemistry, Indiana University, 800 Kirkwood Avenue, Bloomington, Indiana, 47401, USA.
Chem Commun (Camb). 2018 Mar 27;54(26):3270-3273. doi: 10.1039/c7cc09829d.
Ubiquitin confined within nanodroplets was irradiated with a variable-power CO2 laser. Mass spectrometry analysis shows evidence for a protein "melting"-like transition within droplets prior to solvent evaporation and ion formation. Ion mobility spectrometry reveals that structures associated with early steps of denaturation are trapped because of short droplet lifetimes.
用可变功率的二氧化碳激光照射限制在纳米液滴内的泛素。质谱分析表明，在溶剂蒸发和离子形成之前，液滴内存在类似蛋白质“熔化”的转变。离子迁移谱显示，由于液滴寿命短，与变性早期步骤相关的结构被捕获。
