Babenko N A, Storozhenko G V
Fiziol Zh (1994). 2016;62(2):103-9. doi: 10.15407/fz62.02.103.
Age peculiarities of the calorie-restricted diet effects on the contents biologically active sphingo- and glycerolipids were studied in the heart, liver and brain of 3- and 24-month-old rats. Rats were either kept on the ad libitum diet or on a calorie restricted diet (70-80% reduction in total calories) without reduction in essential nutrients. It has been determined that calorie restricted diet decreased the ceramide, sphingomyelin, cardiolipin and phosphatidic acid levels in the all investigated tissues of the rats. At the same time, calorie restriction diet prevented the age-induced ceramide and phosphatidic acid accumulation, ceramide/sphingomyelin ratio elevation, and sphingomyelin and cardiolipin content decrease in the tissues of 24-month-old rats. In addition, tissue specificity of calorierestricted diet effects has been determined. The Elevated levels of cardiolipin and phosphatidic acid were determined in the heart and liver of 24 months-old rats under calorie-restricted diet, in comparison to control animals, whereas in the brain the caloric restriction diet had the opposite effects. These results suggest that calorie-restricted diet may prevent the development of age-associated pathologies due to the modulation of biologically active lipid turnover in the old tissues.
研究了热量限制饮食对3个月和24个月大的大鼠心脏、肝脏和大脑中生物活性鞘脂和甘油磷脂含量的年龄特异性影响。大鼠要么自由进食，要么采用热量限制饮食（总热量减少70 - 80%），但不减少必需营养素。已确定热量限制饮食降低了大鼠所有被研究组织中的神经酰胺、鞘磷脂、心磷脂和磷脂酸水平。同时，热量限制饮食可防止24个月大大鼠组织中年龄诱导的神经酰胺和磷脂酸积累、神经酰胺/鞘磷脂比值升高以及鞘磷脂和心磷脂含量降低。此外，还确定了热量限制饮食影响的组织特异性。与对照动物相比，在热量限制饮食下，24个月大大鼠的心脏和肝脏中心磷脂和磷脂酸水平升高，而在大脑中，热量限制饮食则产生相反的效果。这些结果表明，热量限制饮食可能通过调节老龄组织中生物活性脂质周转来预防与年龄相关的病理发展。
