Spinella D G, Hansen T H, Walsh W D, Behlke M A, Tillinghast J P, Chou H S, Whiteley P J, Kapp J A, Pierce C W, Shevach E M
J Immunol. 1987 Jun 1;138(11):3991-5.
To characterize the T cell receptor repertoire in an immune response in which the Ia and nominal antigenic determinants are defined and limited, we have cloned and sequenced the expressed receptors from four independent, beef insulin-specific T cell lines from C57BL mice. Each of these lines responded to beef but not to the pork insulin, thus defining the nominal antigenic determinant recognized. Furthermore, each of these lines could only be presented antigen by B6 but not mutant B6.C-H-2bm12 antigen-presenting cells, thus defining the requisite Ia recognition or antigen-association site. In spite of this functional similarity in ligand specificity, each of these T cell lines was found to use different V alpha and V beta gene segments. Moreover, structural comparisons of implied protein sequences of each of these receptors showed no stretches of conserved amino acid residues that could be implicated in ligand interaction. However, the V alpha genes used by these four clones appeared considerably more homologous to each other than were their V beta genes.
为了在Ia和名义抗原决定簇已明确且有限的免疫应答中表征T细胞受体库，我们从C57BL小鼠的四个独立的、牛肉胰岛素特异性T细胞系中克隆并测序了表达的受体。这些细胞系中的每一个都对牛肉有反应，但对猪肉胰岛素没有反应，从而确定了所识别的名义抗原决定簇。此外，这些细胞系中的每一个只能由B6抗原呈递细胞而非突变的B6.C-H-2bm12抗原呈递细胞呈递抗原，从而确定了必需的Ia识别或抗原结合位点。尽管在配体特异性方面存在这种功能相似性，但发现这些T细胞系中的每一个都使用不同的Vα和Vβ基因片段。此外，对这些受体中每一个的隐含蛋白质序列进行结构比较，未发现可能与配体相互作用有关的保守氨基酸残基片段。然而，这四个克隆所使用的Vα基因彼此之间的同源性似乎比它们的Vβ基因更高。
