Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, United States of America.
PLoS One. 2018 Mar 14;13(3):e0193601. doi: 10.1371/journal.pone.0193601. eCollection 2018.
THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare the ability of THP-1 macrophages to interact with the facultative intracellular pathogen Salmonella enterica serovar Typhimurium. The results show that THP-1 macrophages differentiated in high concentrations of PMA rapidly died following infection whereas those differentiated in low concentrations of PMA survived and were able to control the intracellular bacteria similar to primary human macrophages.
分化用佛波醇 12-肉豆蔻酸 13-醋酸酯（PMA）的 THP-1 细胞被广泛用作人类巨噬细胞功能和生物学的模型。然而，分化的条件，特别是 PMA 的浓度和处理时间，差异很大。在这里，我们比较了几种分化条件，并比较了 THP-1 巨噬细胞与兼性细胞内病原体鼠伤寒沙门氏菌相互作用的能力。结果表明，感染后高浓度 PMA 分化的 THP-1 巨噬细胞迅速死亡，而低浓度 PMA 分化的 THP-1 巨噬细胞存活下来，并能够像原代人巨噬细胞一样控制细胞内细菌。
