Department of Hematology, The First Affiliated Hospital, Zhejiang University of Medicine, No. 79 Qingchun Road, 310003, Hangzhou, Zhejiang, People's Republic of China.
Key Laboratory of Hematopoietic Malignancies, Hangzhou, Zhejiang Province, People's Republic of China.
J Transl Med. 2018 Mar 14;16(1):68. doi: 10.1186/s12967-018-1445-3.
MicroRNAs are of special interest in cancer research and hold significant promise as diagnostic and prognostic biomarkers for malignant disease. MiR-362-5p have been found to exert both oncogenic and tumor suppressive effects depending highly on the cellular context. The aim of this study was to determine whether the expression of miR-362-5p can be served as a prognostic factor for patients with cytogentically normal acute myeloid leukemia (CN-AML).
We enrolled 224 patients with CN-AML and measured the expression of miR-362-5p by quantitative real time PCR analysis. We classified patients into high and low expression based on the median value. The Cox regression analyses were carried out to assess the prognostic significance of miR-362-5p expression in the context of the well-established predictors. Additionally, microRNA expression profiling were conducted to identify the biological insights between high and low group.
High expressers had older age. High expressers obtained shorter overall survival in the univariate analysis. The independent prognostic value of miR-362-5p remained in the context of the well-established clinical and cytogenetic predictors. Moreover, the prognostic value of miR-362-5p was also validated in an independent cohort of CN-AML. Notably, numerous oncomiRs were also high expressed in high miR-362-5p group.
High miR-362-5p expression was associated with poorer overall survival implicating the oncogenic function in AML development.
microRNAs 在癌症研究中备受关注，作为恶性疾病的诊断和预后生物标志物具有重要意义。miR-362-5p 的作用具有癌基因和肿瘤抑制作用，这高度依赖于细胞环境。本研究旨在确定 miR-362-5p 的表达是否可以作为细胞遗传学正常急性髓系白血病（CN-AML）患者的预后因素。
我们招募了 224 名 CN-AML 患者，并通过定量实时 PCR 分析测量了 miR-362-5p 的表达。我们根据中位数将患者分为高表达和低表达。Cox 回归分析用于评估 miR-362-5p 在既定预测因子背景下的预后意义。此外，还进行了 microRNA 表达谱分析，以确定高表达和低表达组之间的生物学见解。
高表达者年龄较大。在单因素分析中，高表达者的总生存率较短。miR-362-5p 的独立预后价值在既定的临床和细胞遗传学预测因子背景下仍然存在。此外，miR-362-5p 的预后价值也在 CN-AML 的独立队列中得到验证。值得注意的是，在高 miR-362-5p 组中，许多癌基因也高表达。
高 miR-362-5p 表达与总体生存率较差相关，提示其在 AML 发展中具有致癌功能。
