人类肝脏肿瘤标本的漫反射光谱学——迈向使用发光二极管的组织鉴别光学活检针
Diffuse reflectance spectroscopy of human liver tumor specimens - towards a tissue differentiating optical biopsy needle using light emitting diodes.
作者信息
Keller Alina, Bialecki Piotr, Wilhelm Torsten Johannes, Vetter Marcus Klaus
机构信息
Department of Embedded Systems and Biomedical Engineering, Hs Mannheim, University of Applied Sciences, 68163 Mannheim, Germany.
Department of Surgery, University Medical Center Mannheim, University of Heidelberg, 68167 Mannheim, Germany.
出版信息
Biomed Opt Express. 2018 Feb 8;9(3):1069-1081. doi: 10.1364/BOE.9.001069. eCollection 2018 Mar 1.
Abstract
Significant numbers of liver biopsies fail to yield representative tissue samples. This study was conducted to evaluate the ability of LED-based diffuse reflectance spectroscopy to discriminate tumors from liver parenchyma. Ex vivo spectra were acquired from malignant lesions and liver parenchyma of 32 patients who underwent liver resection using a white light source and several LEDs. Integrated spectra of two combined LEDs with emission peaks at 470 nm and 515 nm were classified with 98.4% sensitivity and 99.2% specificity. The promising results could yield to a simple handheld and cost-efficient tool for real-time tissue differentiation implemented in a biopsy needle.
摘要
大量肝脏活检未能获取具有代表性的组织样本。本研究旨在评估基于发光二极管（LED）的漫反射光谱技术区分肿瘤组织与肝实质的能力。利用白光光源和多个发光二极管，采集了32例接受肝切除术患者的恶性病变组织和肝实质的离体光谱。对发射峰位于470纳米和515纳米的两个组合发光二极管的积分光谱进行分类，灵敏度为98.4%，特异性为99.2%。这些有前景的结果可能会产生一种简单、便携且经济高效的工具，用于在活检针中实现实时组织鉴别。
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