Imperial College London, Department of Surgery and Cancer, London, United Kingdom.
Imperial College London, Hamlyn Centre, London, United Kingdom.
J Biomed Opt. 2022 Feb;27(2). doi: 10.1117/1.JBO.27.2.025001.
Diffuse reflectance spectroscopy (DRS) allows discrimination of tissue type. Its application is limited by the inability to mark the scanned tissue and the lack of real-time measurements.
This study aimed to develop a real-time tracking system to enable localization of a DRS probe to aid the classification of tumor and non-tumor tissue.
A green-colored marker attached to the DRS probe was detected using hue-saturation-value (HSV) segmentation. A live, augmented view of tracked optical biopsy sites was recorded in real time. Supervised classifiers were evaluated in terms of sensitivity, specificity, and overall accuracy. A developed software was used for data collection, processing, and statistical analysis.
The measured root mean square error (RMSE) of DRS probe tip tracking was 1.18 ± 0.58 mm and 1.05 ± 0.28 mm for the x and y dimensions, respectively. The diagnostic accuracy of the system to classify tumor and non-tumor tissue in real time was 94% for stomach and 96% for the esophagus.
We have successfully developed a real-time tracking and classification system for a DRS probe. When used on stomach and esophageal tissue for tumor detection, the accuracy derived demonstrates the strength and clinical value of the technique to aid margin assessment in cancer resection surgery.
漫反射光谱（DRS）允许区分组织类型。其应用受到无法标记扫描组织和缺乏实时测量的限制。
本研究旨在开发一种实时跟踪系统，以定位 DRS 探头，帮助对肿瘤和非肿瘤组织进行分类。
使用色调-饱和度-值（HSV）分割检测附着在 DRS 探头上的绿色标记。实时记录跟踪光学活检部位的实时、增强视图。根据灵敏度、特异性和总体准确性评估监督分类器。开发的软件用于数据收集、处理和统计分析。
DRS 探头尖端跟踪的测量均方根误差（RMSE）在 x 和 y 方向上分别为 1.18 ± 0.58 毫米和 1.05 ± 0.28 毫米。该系统实时分类胃和食管组织中肿瘤和非肿瘤组织的诊断准确性分别为 94%和 96%。
我们已经成功开发了一种用于 DRS 探头的实时跟踪和分类系统。当用于胃和食管组织进行肿瘤检测时，所得到的准确性证明了该技术在帮助癌症切除手术中评估边缘方面的优势和临床价值。
