通过从印度比哈尔邦恒河平原中部分离本土细菌来实现砷毒性的可能生物修复。
Possible bioremediation of arsenic toxicity by isolating indigenous bacteria from the middle Gangetic plain of Bihar, India.
作者信息
Satyapal Ghanshyam Kumar, Mishra Santosh Kumar, Srivastava Amrita, Ranjan Rajesh Kumar, Prakash Krishna, Haque Rizwanul, Kumar Nitish
机构信息
Centre for Biological Sciences (Biotechnology), Central University of South Bihar, Patna, Bihar, India.
Centre for Biological Sciences (Life Science), Central University of South Bihar, Patna, Bihar, India.
出版信息
Biotechnol Rep (Amst). 2018 Feb 8;17:117-125. doi: 10.1016/j.btre.2018.02.002. eCollection 2018 Mar.
In middle Gangetic plain, high arsenic concentration is present in water, which causes a significant health risk. Total 48 morphologically distinct arsenite resistant bacteria were isolated from middle Gangetic plain. The minimum inhibitory concentration (MIC) values of arsenite varied widely in the range 1-15 mM of the isolates. On the basis of their MIC, two isolates, AK1 (KY569423) and AK9 (KY569424) were selected. The analysis of the 16S rRNA gene sequence of selected isolates revealed that they are belong to the genus . The AgNO test based microplate method revealed that isolates, AK1 and AK9, have potential in transformation of arsenic species. Further, the presence of and genes in the both isolated strain AK1 and AK9 was confirmed, which play an important role in arsenic bioremediation by arsenite oxidation. Isolated strains also showed heavy metal resistance against Cr(IV), Ni(II), Co(II), Pb(II), Cu(II), Hg(II), Ag(I) and Cd(II).
在恒河中游平原，水中砷浓度很高，这会带来重大健康风险。从恒河中游平原总共分离出48种形态各异的抗亚砷酸盐细菌。分离菌株的亚砷酸盐最低抑菌浓度（MIC）值在1至15毫摩尔范围内差异很大。根据它们的MIC，选择了两个分离株，AK1（KY569423）和AK9（KY569424）。对所选分离株的16S rRNA基因序列分析表明它们属于 属。基于硝酸银测试的微孔板法表明，分离株AK1和AK9在砷形态转化方面具有潜力。此外，在分离菌株AK1和AK9中均证实存在 和 基因，它们通过亚砷酸盐氧化在砷生物修复中起重要作用。分离菌株还显示出对Cr（IV）、Ni（II）、Co（II）、Pb（II）、Cu（II）、Hg（II）、Ag（I）和Cd（II）的重金属抗性。
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