Wargo Matthew J, Rizzo Peter J
Department of Biology, Texas A & M University, College Station, Texas 77843.
J Phycol. 2000 Jun;36(3):584-589. doi: 10.1046/j.1529-8817.2000.99122.x. Epub 2001 Dec 25.
A simple and rapid method for isolation of nuclei from Gymnodinium mikimotoi Miyake et Kominami ex Oda is described along with chemical characterization of the nuclei. The isolated nuclei were completely free of whole cells, 99.96% free of cytoplasmic contamination, and were collected with a yield of 40% from harvested whole cells. Each nucleus contained 47 pg of DNA and the ratio of DNA to acid-soluble proteins to acid-insoluble proteins was 1:0.25:1.21, respectively. SDS electrophoresis of acid-extracted proteins showed one histone-like protein, which we termed HGm, with an apparent molecular mass of 12 kDa. V8 protease digestion analysis of HGm, the histone-like protein from Crypthecodinium cohnii (HCc), and two histone-like proteins from Gymnodinium dorsum, showed that the HGm digestion pattern was more similar to that of HCc than to that of either of the G. dorsum histone-like proteins.
本文描述了一种从米氏裸甲藻（Miyake et Kominami ex Oda）中分离细胞核的简单快速方法，并对细胞核进行了化学表征。分离得到的细胞核完全不含完整细胞，99.96% 无细胞质污染，从收获的完整细胞中收集的产率为40%。每个细胞核含有47 pg的DNA，DNA与酸溶性蛋白和酸不溶性蛋白的比例分别为1:0.25:1.21。酸提取蛋白的SDS电泳显示一种类组蛋白，我们将其命名为HGm，表观分子量为12 kDa。对HGm、来自寇氏隐甲藻（HCc）的类组蛋白以及来自背角裸甲藻的两种类组蛋白进行V8蛋白酶消化分析，结果表明HGm的消化模式与HCc的更相似，而与背角裸甲藻的任何一种类组蛋白的消化模式都不同。
