Mendiola L R, Price C A, Guillard R R
Science. 1966 Sep 30;153(3744):1661-3. doi: 10.1126/science.153.3744.1661.
By means of a medium containing dextran, nuclei were isolated in high yield from cells of Gymnodinium nelsoni, a marine dinoflagellate. Most of the DNA, but less than onetenth of the RNA, of the original cells was recovered in the purified nuclei. The nuclei appeared substantially intact as observed by light or electron microscopy. The isolated nuclei were capable of incorporating tritiated uridine triphosphate into material insoluble in cold acid. The general procedure was found to be applicable also to two species of diatoms.
通过一种含有葡聚糖的培养基，从海洋甲藻尼尔森裸甲藻（Gymnodinium nelsoni）的细胞中高产率地分离出了细胞核。原始细胞中大部分的DNA以及不到十分之一的RNA在纯化的细胞核中得以回收。通过光学显微镜或电子显微镜观察发现，这些细胞核基本保持完整。分离出的细胞核能够将氚标记的尿苷三磷酸掺入冷酸不溶性物质中。人们发现该一般方法也适用于两种硅藻。
