Mniouil Meryem, Fellah Hajiba, Amarir Fatima, Sadak Abderrahim, Et-Touys Abdeslam, Bakri Youssef, Moustachi Aziza, Tassou Fatima Zahraa, Hida Mostapha, Lyagoubi Mohamed, Adlaoui El Bachir, Rhajaoui Mohamed, Sebti Faiza
National Reference Laboratory of Leishmaniasis, Parasitology Department, National Institute of Hygiene, Rabat, Morocco; Laboratory of Zoology and General Biology, Department of Biology, Faculty of Science, Mohammed V University, Rabat, Agdal, Morocco.
Laboratory of Parasitology and Mycology, Institute of Nursing Professions and Health Techniques, Casablanca, Morocco.
Acta Trop. 2018 Jun;182:185-189. doi: 10.1016/j.actatropica.2018.03.007. Epub 2018 Mar 12.
A rapid, sensitive and specific tool for detection of Leishmania infantum infection in Humans would be highly desirable, because it would allow control interventions in endemic areas of visceral leishmaniasis. This study was carried out at the Reference National Laboratory of Leishmaniasis (RNLL) in National Institute of Hygiene (NIH) Morocco, in order to evaluate the diagnostic potential of immunochromatographic dipstick test (ICT) rk39 in Moroccan suspected VL patients. A total of 49 admitted patients with strong clinical suspicion of VL and 40 healthy controls were investigated for the performance of the ICT rk39. Bone marrow smears were examined for microscopic detection of Leishmania amastigotes obtained from the admitted patients. Only PCR and smear positive cases were considered as gold standard as well as confirmed cases of VL. Out of 49 suspected patients, twenty four (48.9%) were found PCR and smear-positive and twenty three (46.9%) were positive for ICT rk39. Voluntary healthy controls, which included twenty persons from the endemic zone and twenty from non-endemic zone of VL, were found all negative for the strip test. The sensitivity in sera was 75% by ELISA and 87.5% by IFAT, compared with 95.8% for ICT rk39. Specificity was 95.8%, with both tests ELISA and IFAT, and 100% by ICT rk39 respectively. Present study findings again reinforce that the ICT rk39 is a simple, reliable and easy-to-perform non-invasive diagnostic tool for visceral leishmaniasis in the endemic area of Morocco.
非常需要一种快速、灵敏且特异的工具来检测人类中的婴儿利什曼原虫感染，因为这将有助于在内脏利什曼病流行地区进行控制干预。本研究在摩洛哥国家卫生研究所的国家利什曼病参考实验室（RNLL）开展，目的是评估免疫层析试纸条检测（ICT）rk39在摩洛哥疑似内脏利什曼病患者中的诊断潜力。对49名临床高度怀疑内脏利什曼病的住院患者和40名健康对照者进行了ICT rk39检测性能的调查。对住院患者的骨髓涂片进行检查，以显微镜检测利什曼原虫无鞭毛体。仅将PCR和涂片阳性病例以及确诊的内脏利什曼病病例视为金标准。在49名疑似患者中，24例（48.9%）PCR和涂片呈阳性，23例（46.9%）ICT rk39呈阳性。自愿健康对照者包括20名来自内脏利什曼病流行区和20名来自非流行区的人，试纸条检测均为阴性。ELISA检测血清的敏感性为75%，IFAT检测为87.5%，而ICT rk39检测为95.8%。ELISA和IFAT检测的特异性分别为95.8%，ICT rk39检测为100%。本研究结果再次证实，ICT rk39是摩洛哥流行地区一种简单、可靠且易于操作的非侵入性内脏利什曼病诊断工具。
