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1
Whole-Genome Sequencing of D75 and D76 Confirms Safety and Probiotic Potential.D75和D76的全基因组测序证实了其安全性和益生菌潜力。
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Genomic analysis of WHH1689 reveals its probiotic properties and stress resistance.对WHH1689的基因组分析揭示了其益生菌特性和抗逆性。
Food Sci Nutr. 2019 Jan 28;7(2):844-857. doi: 10.1002/fsn3.934. eCollection 2019 Feb.

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NCBI prokaryotic genome annotation pipeline.美国国立生物技术信息中心原核生物基因组注释管道
Nucleic Acids Res. 2016 Aug 19;44(14):6614-24. doi: 10.1093/nar/gkw569. Epub 2016 Jun 24.
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Precision methylome characterization of Mycobacterium tuberculosis complex (MTBC) using PacBio single-molecule real-time (SMRT) technology.利用PacBio单分子实时（SMRT）技术对结核分枝杆菌复合群（MTBC）进行精确甲基化组表征。
Nucleic Acids Res. 2016 Jan 29;44(2):730-43. doi: 10.1093/nar/gkv1498. Epub 2015 Dec 23.
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PacBio Sequencing and Its Applications.PacBio测序技术及其应用。
Genomics Proteomics Bioinformatics. 2015 Oct;13(5):278-89. doi: 10.1016/j.gpb.2015.08.002. Epub 2015 Nov 2.
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Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data.非杂交、基于长读长 SMRT 测序数据的完成微生物基因组组装。
Nat Methods. 2013 Jun;10(6):563-9. doi: 10.1038/nmeth.2474. Epub 2013 May 5.
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Complete genome sequence of Lactobacillus helveticus R0052, a commercial probiotic strain.瑞士乳杆菌 R0052 全基因组序列，一种商业益生菌菌株。
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Genome sequence of Lactobacillus helveticus, an organism distinguished by selective gene loss and insertion sequence element expansion.瑞士乳杆菌的基因组序列，该生物体以选择性基因丢失和插入序列元件扩增为特征。
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Genomic insights that advance the species definition for prokaryotes.推动原核生物物种定义的基因组学见解。
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Development of a single-tube, cell lysis-based, genus-specific PCR method for rapid identification of mycobacteria: optimization of cell lysis, PCR primers and conditions, and restriction pattern analysis.开发一种基于单管细胞裂解的属特异性PCR方法用于快速鉴定分枝杆菌：细胞裂解、PCR引物和条件的优化以及限制性图谱分析。
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A simple method for the isolation of chromosomal DNA from gram positive or acid-fast bacteria.一种从革兰氏阳性菌或抗酸菌中分离染色体DNA的简单方法。
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益生菌乳酸菌D75和D76的全基因组序列

Complete Genome Sequences of the Probiotic Lactic Acid Bacteria D75 and D76.

作者信息

Toropov V A, Vakhitov T Y, Shalaeva O N, Roshchina E K, Sitkin S I

机构信息

State Research Institute of Highly Pure Biopreparations of the FMBA of the Russian Federation, St. Petersburg, Russia.

State Research Institute of Highly Pure Biopreparations of the FMBA of the Russian Federation, St. Petersburg, Russia

出版信息

Genome Announc. 2018 Mar 15;6(11):e01552-17. doi: 10.1128/genomeA.01552-17.

DOI:10.1128/genomeA.01552-17
PMID:29545307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5854786/
Abstract

D75 and D76 were isolated from the intestinal tract of a healthy child. Both strains possess symbiotic, probiotic, and antagonistic activities. We have sequenced and annotated the whole genomes of D75 and D76 and have conducted a preliminary genome comparative analysis.

摘要

D75和D76是从一名健康儿童的肠道中分离出来的。这两种菌株都具有共生、益生菌和拮抗活性。我们已经对D75和D76的全基因组进行了测序和注释，并进行了初步的基因组比较分析。