通过对cDNA克隆的分析对人补体调节蛋白I因子的一级氨基酸序列进行表征。
Characterization of primary amino acid sequence of human complement control protein factor I from an analysis of cDNA clones.
作者信息
Catterall C F, Lyons A, Sim R B, Day A J, Harris T J
出版信息
Biochem J. 1987 Mar 15;242(3):849-56. doi: 10.1042/bj2420849.
Abstract
A cDNA clone of the mRNA coding for the human complement system control protein Factor I has been isolated. The predicted amino acid sequence obtained from the DNA sequence demonstrates a protein consisting of a heavy chain (Mr 35,400) linked to a light chain (Mr 27,600), both of which contain three sites for N-linked glycosylation. The light chain has clear homology with other serine proteinases, most notably in the region of the catalytically active and structurally important amino acids and shares some of the features characteristic of the plasminogen activators. The heavy chain has a clear 'mosaic' nature typical of the plasma serine proteinases; in particular it contains class A and class B LDL (low-density lipoprotein) receptor repeats with conserved cysteine residues similar to those found in other complement proteins.
摘要
编码人补体系统调控蛋白I因子的mRNA的cDNA克隆已被分离出来。从DNA序列获得的预测氨基酸序列显示，该蛋白质由一条重链（分子量35400）和一条轻链（分子量27600）相连组成，二者均含有三个N-连接糖基化位点。轻链与其他丝氨酸蛋白酶具有明显的同源性，最显著的是在催化活性和结构重要氨基酸区域，并且具有一些纤溶酶原激活剂的特征。重链具有血浆丝氨酸蛋白酶典型的明显“镶嵌”性质；特别是它含有A类和B类低密度脂蛋白（LDL）受体重复序列，带有与其他补体蛋白中发现的类似的保守半胱氨酸残基。
相似文献
1
Characterization of primary amino acid sequence of human complement control protein factor I from an analysis of cDNA clones.通过对cDNA克隆的分析对人补体调节蛋白I因子的一级氨基酸序列进行表征。
Biochem J. 1987 Mar 15;242(3):849-56. doi: 10.1042/bj2420849.
2
Human complement factor I: analysis of cDNA-derived primary structure and assignment of its gene to chromosome 4.
J Biol Chem. 1987 Jul 25;262(21):10065-71.
3
Cloning of cDNA coding for the beta chain of human complement component C4b-binding protein: sequence homology with the alpha chain.
Proc Natl Acad Sci U S A. 1990 Feb;87(3):1183-7. doi: 10.1073/pnas.87.3.1183.
4
Molecular cloning and characterization of the cDNA coding for C4b-binding protein, a regulatory protein of the classical pathway of the human complement system.人补体系统经典途径调节蛋白C4b结合蛋白编码cDNA的分子克隆与特性分析
Biochem J. 1985 Aug 15;230(1):133-41. doi: 10.1042/bj2300133.
5
Cloning of a cDNA coding for human factor V, a blood coagulation factor homologous to factor VIII and ceruloplasmin.编码人凝血因子V的cDNA的克隆，凝血因子V是一种与凝血因子VIII和铜蓝蛋白同源的凝血因子。
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6800-4. doi: 10.1073/pnas.83.18.6800.
6
Precerebellin is a cerebellum-specific protein with similarity to the globular domain of complement C1q B chain.小脑前体蛋白是一种小脑特异性蛋白，与补体C1q B链的球状结构域相似。
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):1069-73. doi: 10.1073/pnas.88.3.1069.
7
CNBr cleavage of the light chain of human complement factor I and alignment of the fragments.人补体因子I轻链的溴化氰裂解及片段比对
Biochem J. 1986 Jan 15;233(2):339-45. doi: 10.1042/bj2330339.
8
Analysis of cDNA clones encoding the entire B-26 region of human apolipoprotein B.编码人载脂蛋白B完整B-26区域的cDNA克隆分析。
Proc Natl Acad Sci U S A. 1986 Aug;83(15):5678-82. doi: 10.1073/pnas.83.15.5678.
9
Cloning of the alpha chain of human platelet glycoprotein Ib: a transmembrane protein with homology to leucine-rich alpha 2-glycoprotein.人血小板糖蛋白 Ib α 链的克隆：一种与富含亮氨酸的 α2-糖蛋白具有同源性的跨膜蛋白。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5615-9. doi: 10.1073/pnas.84.16.5615.
10
Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure.编码人癌胚抗原的cDNA克隆的分离与鉴定揭示了一种高度保守的重复结构。
Proc Natl Acad Sci U S A. 1987 May;84(9):2960-4. doi: 10.1073/pnas.84.9.2960.
引用本文的文献
1
Rare Dysfunctional Genetic Variants and Progression to Advanced Age-Related Macular Degeneration.罕见的功能失调性基因变异与进展为晚期年龄相关性黄斑变性
Ophthalmol Sci. 2022 Dec 30;3(2):100265. doi: 10.1016/j.xops.2022.100265. eCollection 2023 Jun.
2
Rapid genome sequencing identifies novel variants in complement factor I.快速基因组测序鉴定补体因子 I 中的新型变异体。
Cold Spring Harb Mol Case Stud. 2022 Dec 28;8(7). doi: 10.1101/mcs.a006239. Print 2022 Dec.
3
Kidney diseases.肾脏疾病。
Immunol Rev. 2023 Jan;313(1):239-261. doi: 10.1111/imr.13167. Epub 2022 Nov 12.
4
A Personal Tribute to Robert B. Sim with Reflections on Our Work Together on Factor H.向罗伯特·B·西姆致敬——纪念我们共同研究因子 H 的工作
Viruses. 2021 Jun 28;13(7):1256. doi: 10.3390/v13071256.
5
The Challenges and Promise of Complement Therapeutics for Ocular Diseases.补体治疗眼部疾病的挑战与前景。
Front Immunol. 2019 May 15;10:1007. doi: 10.3389/fimmu.2019.01007. eCollection 2019.
6
Association between vitamin D status and age-related macular degeneration by genetic risk.维生素D状态与年龄相关性黄斑变性的遗传风险之间的关联。
JAMA Ophthalmol. 2015 Oct;133(10):1171-9. doi: 10.1001/jamaophthalmol.2015.2715.
7
Acquisition of complement inhibitor serine protease factor I and its cofactors C4b-binding protein and factor H by Prevotella intermedia.中间普雷沃菌对补体抑制剂丝氨酸蛋白酶因子 I 及其辅助因子 C4b 结合蛋白和因子 H 的获取。
PLoS One. 2012;7(4):e34852. doi: 10.1371/journal.pone.0034852. Epub 2012 Apr 13.
8
Analysis of binding sites on complement factor I using artificial N-linked glycosylation.利用人工 N 连接糖基化分析补体因子 I 的结合位点。
J Biol Chem. 2012 Apr 20;287(17):13572-83. doi: 10.1074/jbc.M111.326298. Epub 2012 Mar 5.
9
Characterization of shark complement factor I gene(s): genomic analysis of a novel shark-specific sequence.鲨鱼补体因子I基因的特征分析：一种新型鲨鱼特异性序列的基因组分析
Mol Immunol. 2009 Jul;46(11-12):2299-308. doi: 10.1016/j.molimm.2009.04.002. Epub 2009 May 7.
10
Molecular basis of complement factor I (CFI) polymorphism: one of two polymorphic suballeles responsible for CFI A is Japanese-specific.
J Hum Genet. 2008;53(11-12):1016-1021. doi: 10.1007/s10038-008-0337-4. Epub 2008 Sep 30.
本文引用的文献
1
Oligonucleotide directed mutagenesis of the human beta-globin gene: a general method for producing specific point mutations in cloned DNA.人β-珠蛋白基因的寡核苷酸定向诱变：在克隆DNA中产生特定位点突变的通用方法。
Nucleic Acids Res. 1981 Aug 11;9(15):3647-56. doi: 10.1093/nar/9.15.3647.
2
Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.DNA测序鸟枪法产生的凝胶读数数据的计算机处理自动化。
Nucleic Acids Res. 1982 Aug 11;10(15):4731-51. doi: 10.1093/nar/10.15.4731.
3
The C3b inactivator of the human complement system: homology with serine proteases.人类补体系统的C3b灭活因子：与丝氨酸蛋白酶的同源性。
FEBS Lett. 1981 Nov 16;134(2):147-50. doi: 10.1016/0014-5793(81)80588-1.
4
Structure, specificity and localization of the serine proteases of connective tissue.结缔组织丝氨酸蛋白酶的结构、特异性及定位
FEBS Lett. 1980 Jun 2;114(2):189-96. doi: 10.1016/0014-5793(80)81112-4.
5
Characterization of a cDNA coding for human protein C.编码人蛋白C的cDNA的特性分析。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4766-70. doi: 10.1073/pnas.81.15.4766.
6
Characterization of a cDNA coding for human factor X.编码人凝血因子X的cDNA的特性分析
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3699-702. doi: 10.1073/pnas.81.12.3699.
7
The structural basis of the multiple forms of human complement component C4.人类补体成分C4多种形式的结构基础。
Cell. 1984 Apr;36(4):907-14. doi: 10.1016/0092-8674(84)90040-0.
8
The evolutionary relationship of the enzymes involved in blood coagulation and hemostasis.
Ann N Y Acad Sci. 1981;370:511-27. doi: 10.1111/j.1749-6632.1981.tb29759.x.
9
Mouse 7S nerve growth factor: complete sequence of a cDNA coding for the alpha-subunit precursor and its relationship to serine proteases.小鼠7S神经生长因子：编码α亚基前体的cDNA完整序列及其与丝氨酸蛋白酶的关系。
Biochemistry. 1984 Dec 4;23(25):5997-6002. doi: 10.1021/bi00320a015.
10
Compilation of published signal sequences.已发表信号序列的汇编。
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64. doi: 10.1093/nar/12.13.5145.
Zotero 插件