人补体因子I轻链的溴化氰裂解及片段比对
CNBr cleavage of the light chain of human complement factor I and alignment of the fragments.
作者信息
Yuan J M, Hsiung L M, Gagnon J
出版信息
Biochem J. 1986 Jan 15;233(2):339-45. doi: 10.1042/bj2330339.
Abstract
The light chain and heavy chain of reduced and alkylated human complement Factor I were purified by high-pressure gel-permeation chromatography. CNBr cleavage of Factor I light chain yielded four major fragments, which were purified by gel filtration. N-Terminal sequence analysis of the CNBr-cleavage fragments allowed identification of 200 of the approx. 240 amino acid residues of the light chain. An alignment is proposed, based on sequence analysis of peptides obtained after cleavage at arginine residues of the light chain and on homology of the sequence determined with that of other serine proteinases. The sequence around the active-site serine residue was established and three potential attachment sites for carbohydrate moieties were identified.
摘要
通过高压凝胶渗透色谱法纯化还原和烷基化的人补体I因子的轻链和重链。用溴化氰裂解I因子轻链产生四个主要片段，通过凝胶过滤对其进行纯化。对溴化氰裂解片段进行N端序列分析，从而鉴定出轻链约240个氨基酸残基中的200个。基于轻链精氨酸残基裂解后所得肽段的序列分析以及所确定序列与其他丝氨酸蛋白酶序列的同源性，提出了一种比对。确定了活性位点丝氨酸残基周围的序列，并鉴定出三个潜在的碳水化合物部分附着位点。
相似文献
1
CNBr cleavage of the light chain of human complement factor I and alignment of the fragments.人补体因子I轻链的溴化氰裂解及片段比对
Biochem J. 1986 Jan 15;233(2):339-45. doi: 10.1042/bj2330339.
2
The catalytic chain of human complement subcomponent C1r. Purification and N-terminal amino acid sequences of the major cyanogen bromide-cleavage fragments.人补体亚成分C1r的催化链。主要溴化氰裂解片段的纯化及N端氨基酸序列
Biochem J. 1982 Jan 1;201(1):49-59. doi: 10.1042/bj2010049.
3
The serine proteinase chain of human complement component C1s. Cyanogen bromide cleavage and N-terminal sequences of the fragments.人补体成分C1s的丝氨酸蛋白酶链。溴化氰裂解及片段的N端序列。
Biochem J. 1983 Dec 1;215(3):565-71. doi: 10.1042/bj2150565.
4
Amino acid sequence of the Bb fragment from human complement Factor B. Alignment of the cyanogen bromide-cleavage peptides.人补体因子B的Bb片段的氨基酸序列。溴化氰裂解肽的比对。
Biochem J. 1983 Jan 1;209(1):51-60. doi: 10.1042/bj2090051.
5
Isolation, characterization and N-terminal sequences of the CNBr-cleavage peptides from human complement Factor B. Localization of a free thiol group and a sequence defining the site cleaved by factor D.人补体因子B中溴化氰裂解肽段的分离、特性鉴定及N端序列。游离巯基的定位以及定义被因子D裂解位点的序列。
Biochem J. 1982 Mar 1;201(3):555-67. doi: 10.1042/bj2010555.
6
Characterization of the cyanogen bromide fragments of the beta chain of human haptoglobin.人触珠蛋白β链溴化氰片段的特性分析
Biochemistry. 1976 Nov 30;15(24):5326-36. doi: 10.1021/bi00669a020.
7
Human complement component C1s. Partial sequence determination of the heavy chain and identification of the peptide bond cleaved during activation.人补体成分C1s。重链部分序列测定及激活过程中裂解的肽键鉴定。
Eur J Biochem. 1986 Apr 1;156(1):49-57. doi: 10.1111/j.1432-1033.1986.tb09546.x.
8
Primary structure of the A chain of human complement-classical-pathway enzyme C1r. N-terminal sequences and alignment of autolytic fragments and CNBr-cleavage peptides.人补体经典途径酶C1r A链的一级结构。N端序列以及自溶片段和溴化氰裂解肽的比对。
Biochem J. 1985 Jan 1;225(1):135-42. doi: 10.1042/bj2250135.
9
Amino acid sequence of the Bb fragment from complement Factor B. Sequence of the major cyanogen bromide-cleavage peptide (CB-II) and completion of the sequence of the Bb fragment.补体因子B的Bb片段的氨基酸序列。主要溴化氰裂解肽（CB-II）的序列以及Bb片段序列的完成。
Biochem J. 1983 Jan 1;209(1):61-70. doi: 10.1042/bj2090061.
10
Chemical characterization of cyanogen bromide fragments from the beta-chain of human complement factor C3.人补体因子C3β链溴化氰片段的化学特性分析
FEBS Lett. 1984 Apr 9;169(1):57-62. doi: 10.1016/0014-5793(84)80289-6.
引用本文的文献
1
Production of a de-novo designed antimicrobial peptide in Nicotiana benthamiana.在本氏烟中生产一种从头设计的抗菌肽。
Plant Mol Biol. 2013 Feb;81(3):259-72. doi: 10.1007/s11103-012-9996-9. Epub 2012 Dec 16.
2
Rat complement factor I: molecular cloning, sequencing and expression in tissues and isolated cells.大鼠补体因子I：分子克隆、测序及在组织和分离细胞中的表达。
Immunology. 1999 Nov;98(3):464-74. doi: 10.1046/j.1365-2567.1999.00886.x.
3
Characterization of primary amino acid sequence of human complement control protein factor I from an analysis of cDNA clones.通过对cDNA克隆的分析对人补体调节蛋白I因子的一级氨基酸序列进行表征。
Biochem J. 1987 Mar 15;242(3):849-56. doi: 10.1042/bj2420849.
本文引用的文献
1
2
The physiological breakdown of the third component of human complement.人类补体第三成分的生理分解。
Mol Immunol. 1980 Jan;17(1):9-20. doi: 10.1016/0161-5890(80)90119-4.
3
Purification of human C3b inactivator by monoclonal-antibody affinity chromatography.用单克隆抗体亲和层析法纯化人C3b灭活剂。
Biochem J. 1982 Apr 1;203(1):293-8. doi: 10.1042/bj2030293.
4
Isolation, characterization and N-terminal sequences of the CNBr-cleavage peptides from human complement Factor B. Localization of a free thiol group and a sequence defining the site cleaved by factor D.人补体因子B中溴化氰裂解肽段的分离、特性鉴定及N端序列。游离巯基的定位以及定义被因子D裂解位点的序列。
Biochem J. 1982 Mar 1;201(3):555-67. doi: 10.1042/bj2010555.
5
The C3b inactivator of the human complement system: homology with serine proteases.人类补体系统的C3b灭活因子：与丝氨酸蛋白酶的同源性。
FEBS Lett. 1981 Nov 16;134(2):147-50. doi: 10.1016/0014-5793(81)80588-1.
6
The proteolytic activation systems of complement.补体的蛋白水解激活系统。
Annu Rev Biochem. 1981;50:433-64. doi: 10.1146/annurev.bi.50.070181.002245.
7
Human complement component C4. Structural studies on the fragments derived from C4b by cleavage with C3b inactivator.人补体成分C4。关于用C3b灭活剂裂解C4b产生的片段的结构研究。
Biochem J. 1981 Nov 1;199(2):351-7. doi: 10.1042/bj1990351.
8
The structural basis of the multiple forms of human complement component C4.人类补体成分C4多种形式的结构基础。
Cell. 1984 Apr;36(4):907-14. doi: 10.1016/0092-8674(84)90040-0.
9
Pattern of degradation of human complement fragment, C3b.人补体片段C3b的降解模式
FEBS Lett. 1981 Sep 14;132(1):55-60. doi: 10.1016/0014-5793(81)80426-7.
10
Purification of the human complement control protein C3b inactivator.人补体调节蛋白C3b灭活剂的纯化
Biochem J. 1980 Oct 1;191(1):173-82. doi: 10.1042/bj1910173.
Zotero 插件