Zhang Yong-Hui, Pan Lian-Hong, Pang Yi, Yang Jin-Xin, Lv Meng-Jia, Liu Feng, Qu Xue-Feng, Chen Xin-Xin, Gong Hua-Jun, Liu Dan, Wei Yong
Department of Basic Medical Science, Chongqing Three Gorges Medical College, Chongqing 404120, P.R. China.
Chongqing Engineering Research Center of Antitumor Natural Drugs, Chongqing 404120, P.R. China.
Exp Ther Med. 2018 Apr;15(4):3495-3500. doi: 10.3892/etm.2018.5861. Epub 2018 Feb 12.
Growth differentiation factor 11 (GDF11), also known as bone morphogenetic protein 11, a member of the transforming growth factor-β superfamily, has been reported to be involved in colorectal cancer. However, the roles of GDF11 in Chinese patients with liver cancer and the underlying mechanisms have remained elusive. The present study assessed the expression of GDF11 in 10 paired samples of cancerous and normal tissues from Chinese liver cancer patients. The results indicated that the expression of GDF11 was significantly lower in cancerous tissues than in normal tissues. , the expression of GDF11 was reduced in a panel of liver cancer cell lines compared with that in a normal liver cell line at the mRNA and protein level. Treatment with GDF11 reduced the viability of HepG2 for up to 72 h and GDF11 treatment reduced the viability of SMMC-7721 after 48 and 72 h. Furthermore, GDF11 activated Smad2/3 signaling in HepG2 cells. In conclusion, GDF11 has a tumor suppressor role in liver cancer, exerts its effects through Smad2/3 signaling and may serve as a novel tumor marker in liver cancer diagnosis.
生长分化因子11（GDF11），也被称为骨形态发生蛋白11，是转化生长因子-β超家族的成员之一，据报道其与结直肠癌有关。然而，GDF11在中国肝癌患者中的作用及其潜在机制仍不清楚。本研究评估了GDF11在10对中国肝癌患者癌组织和正常组织样本中的表达。结果表明，GDF11在癌组织中的表达明显低于正常组织。此外，与正常肝细胞系相比，在一组肝癌细胞系中，GDF11在mRNA和蛋白质水平上的表达均降低。用GDF11处理可使HepG2细胞活力降低长达72小时，而GDF11处理48小时和72小时后可降低SMMC-7721细胞的活力。此外，GDF11激活了HepG2细胞中的Smad2/3信号通路。总之，GDF11在肝癌中具有肿瘤抑制作用，通过Smad2/3信号通路发挥作用，可能作为肝癌诊断中的一种新型肿瘤标志物。
