Kumar Vivek, Francis Antonio, Avery Pasco B, McKenzie Cindy L, Osborne Lance S
Mid-Florida Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Apopka, FL.
United States Department of Agriculture, Agricultural Research Services, Fort Pierce, FL.
J Econ Entomol. 2018 May 28;111(3):1069-1079. doi: 10.1093/jee/toy056.
Rugose spiraling whitefly (RSW), Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is a new invasive whitefly pest in the Florida landscape, known to feed on a wide range of plants including palms, woody ornamentals, shrubs, and fruits. With the objective to find an alternative to neonicotinoid insecticides, and develop an ecofriendly management program for RSW, in the current study we evaluated the efficacy of a biopesticide containing the entomopathogenic fungi, Isaria fumosorosea Wize (Hypocreales: Clavicipitaceae), and an insect growth regulator buprofezin applied alone and in combination under laboratory and field conditions. Before assessing the two products, their compatibility was studied at six different concentrations of buprofezin. No significant inhibitive effect of buprofezin was observed on I. fumosorosea spore germination, and the average linear growth of the colony measured 14 d postinoculation. Under laboratory conditions, I. fumosorosea treatments (alone or mixed with buprofezin) provided higher RSW mortality than buprofezin alone. However, in both outdoor cage studies, the efficacy of buprofezin treatments (alone or mixed with I. fumosorosea) was higher than I. fumosorosea alone. A significant reduction in RSW population was reported for more than 5 wk in buprofezin alone and more than 7 wk in the combination treatments. In fall of 2014 and summer of 2015, the mean whitefly mortality observed during the 10-wk assessment period was 52.4 and 42.1% for I. fumosorosea, 79.6 and 79.0% for buprofezin, and 87.6 and 84.3% in mixed treatments, respectively. Results suggest that buprofezin can offer an effective alternate in the battle against invasive whiteflies such as RSW in Florida ecosystems, either as a stand-alone strategy or in an integrated approach.
皱纹螺旋粉虱（RSW），即 Aleurodicus rugioperculatus Martin（半翅目：粉虱科），是佛罗里达景观中一种新的入侵性粉虱害虫，已知以多种植物为食，包括棕榈树、木本观赏植物、灌木和水果。为了寻找新烟碱类杀虫剂的替代品，并为皱纹螺旋粉虱制定一个生态友好型管理方案，在本研究中，我们评估了一种含有昆虫病原真菌玫烟色棒束孢（Isaria fumosorosea Wize，肉座菌目：麦角菌科）的生物农药以及一种昆虫生长调节剂噻嗪酮在实验室和田间条件下单独使用及联合使用的效果。在评估这两种产品之前，研究了噻嗪酮在六种不同浓度下与玫烟色棒束孢的兼容性。未观察到噻嗪酮对玫烟色棒束孢孢子萌发有显著抑制作用，且在接种后14天测量的菌落平均线性生长情况良好。在实验室条件下，玫烟色棒束孢处理（单独或与噻嗪酮混合）比单独使用噻嗪酮导致更高的皱纹螺旋粉虱死亡率。然而，在两项室外网室研究中，噻嗪酮处理（单独或与玫烟色棒束孢混合）的效果高于单独使用玫烟色棒束孢。单独使用噻嗪酮时，皱纹螺旋粉虱种群数量在5周多的时间里显著减少，联合处理则在7周多的时间里显著减少。在2014年秋季和2015年夏季，在为期10周的评估期内观察到的粉虱平均死亡率，玫烟色棒束孢分别为52.4%和42.1%，噻嗪酮分别为79.6%和79.0%，混合处理分别为87.6%和84.3%。结果表明，噻嗪酮无论是作为单独的策略还是综合方法，都可以在对抗佛罗里达生态系统中诸如皱纹螺旋粉虱等入侵性粉虱的战斗中提供一种有效的替代方案。
