Anel-López Luis, Garcia-Álvarez Olga, Tarantini Tatiana, Del Olmo David, Ortiz Jose Antonio, Ledda Sergio, Martinez Emilio Arsenio, Soler Ana J, Roca Jordi, Fernández Santos María R, Vázquez Juan Maria, Parrilla Inmaculada, Garde Jose Julián
ITRA-ULE, Departamento de Medicina, Cirugía y Anatomía Veterinaria, University of León, Spain.
Biomedical Center, School of Medicine in Pilsen, Charles University in Prague, alej Svobody 1655/76, 323 00 Pilsen, Czech Republic; SaBio IREC (CSIC-UCLM- JCCM), Albacete, Spain.
Theriogenology. 2018 Jun;113:171-175. doi: 10.1016/j.theriogenology.2018.03.005. Epub 2018 Mar 9.
The aim of this study was to assess the effect of insemination timing on pregnancy rates in red deer (Cervus elaphus) when using sex-sorted sperm samples. Semen was collected by electroejaculation from 8 mature stags and processed to obtain: Conventional samples, following standard freezing procedures for commercial purposes; Control sorted samples, diluted and handled as per sorted samples but without being submitted to the sorter passage; and Y Sex Sorted (YSS) samples. Hinds were synchronized via intravaginal CIDR (Controlled Internal Drug Release) placement and given eCG (Folligon PMSG Serum Gonadotrophin) on day 12, upon CIDR removal. They were then inseminated with one of each sperm treatment, at the following post-eCG intervals: I_1, 55:01-55:30 h; I_2, 55:31-56:00 h; I_3, 56:01-56:30 h; or, I_4, 56:31-57:00 h. Pregnancy rates were assessed at parturition. Average pregnancy rates were highest (P < 0.05) for Conventional samples (77.6%), but similar between YSS (49.8%) and Control sorted (51.3%) samples. However, when insemination interval was taken into account, pregnancy rates within the YSS group, pregnancy rates were 80 and 83.1% for I_1 and I_2, respectively were obtained. Notably, these rates were similar (P > 0.05) to the average pregnancy rates obtained with Conventional samples (77.6%). As expected, YSS sperm yielded 94% male offspring contrasting with the 57% males obtained with Conventional and Control sorted samples. Our findings support the importance of developing specific insemination timing protocols to improve pregnancy rates when using frozen-thawed sex-sorted sperm. These findings provide the foundation for further investigations in order to determine why the YSS sperm are able to fertilize the oocyte in a shorter period of time than the conventional samples.
本研究的目的是评估在使用性别分选精子样本时，授精时间对马鹿（ Cervus elaphus ）妊娠率的影响。通过电刺激射精从8头成年雄鹿采集精液，并进行处理以获得：用于商业目的的按照标准冷冻程序处理的常规样本；对照分选样本，按分选样本进行稀释和处理，但未经过分选仪通道；以及Y染色体性别分选（YSS）样本。通过阴道内放置CIDR（控制内部药物释放）装置使母鹿同步发情，并在第12天取出CIDR时给予eCG（Folligon PMSG血清促性腺激素）。然后在eCG注射后的以下时间间隔，用每种精子处理样本之一对母鹿进行授精：I_1，55:01 - 55:30小时；I_2，55:31 - 56:00小时；I_3，56:01 - 56:30小时；或I_4，56:31 - 57:00小时。在分娩时评估妊娠率。常规样本的平均妊娠率最高（P < 0.05），为77.6%，但YSS样本（49.8%）和对照分选样本（51.3%）的妊娠率相似。然而，考虑授精间隔时，YSS组内，I_1和I_2的妊娠率分别为80%和83.1%。值得注意的是，这些比率与常规样本获得的平均妊娠率（77.6%）相似（P > 0.05）。正如预期的那样，YSS精子产生了94%的雄性后代，而常规样本和对照分选样本产生的雄性后代为57%。我们的研究结果支持了制定特定授精时间方案以提高使用冻融性别分选精子时妊娠率的重要性。这些发现为进一步研究奠定了基础，以便确定为什么YSS精子能够比常规样本在更短的时间内使卵母细胞受精。
