Korzekwa Anna J, Kordan Władysław, Kotlarczyk Angelika M, Kozdrowski Roland
Department of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Str., 10-748 Olsztyn, Poland.
Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland.
Animals (Basel). 2020 Nov 19;10(11):2148. doi: 10.3390/ani10112148.
The aim was to estimate the effective pharmacological method of the estrous cycle synchronization by checking the effects of synchronization by measurement of progesterone (P4) and 17-beta estradiol (E2) concentration by RIA and artificial insemination. The experiment was performed at the red deer farm in Rudzie (North-East Poland; 3 year's old). The herd (N = 14) was kept away from bulls and was divided in two groups of seven animals. In the Group I, CIDR insert (0.3 g of P4) was applicated intravaginally for 12 days; a second insert replaced the first one for the next 12 days, and next 200 IU of equine chorionic gonadotropin (eCG) was injected intramuscularly (Folligon). Estrus was expected 48 h after eCG injection. In the Group II, Chronogest sponge (20 mg of flugestone acetate) was applicated intravaginally and after 7 days replaced with second chronogest sponge for 7 days. After removing the sponge, on the same day eCG was injected and estrus was expected after 48 h. Artificial insemination was provided with frozen-thawed semen twice: 12 and 24 h after expected estrus. The peripheral blood from the jugular vein was collected each time when the inserts or sponge were applicated and 40 days after insemination. The concentration of P4 and E2 in plasma was measured by RIA. The effectiveness of insemination was monitored by glycoproteins determination and observed by the number of calves born. Two pregnancies were confirmed in Group I and five in Group II based on PAG concentration. One newborn was observed in Group I and five in Group II. Both methods of synchronization are effective in hinds based on the received profile of steroids. Although the sponge shape in case of chronogest is better comparing with CIDR, which was not completely deposited in the vagina of hind, potentially leads to bacteria inflammation, and it disturbs the rightful endocrine regulation. Moreover, pregnancy rate and hormone responsiveness were better in Group II.
目的是通过放射免疫分析法（RIA）测量孕酮（P4）和17-β雌二醇（E2）浓度以及人工授精来检查发情周期同步化的效果，从而评估发情周期同步化的有效药理学方法。实验在鲁德齐（波兰东北部；3岁）的马鹿养殖场进行。鹿群（N = 14）与公牛隔离，并分为两组，每组7只动物。在第一组中，将含有0.3克P4的阴道孕酮释放装置（CIDR）经阴道放置12天；第二个装置在接下来的12天替换第一个装置，然后肌肉注射200国际单位的马绒毛膜促性腺激素（eCG）（Folligon）。预计在注射eCG后48小时发情。在第二组中，将含有20毫克醋酸氟孕酮的Chronogest海绵经阴道放置，7天后用第二个Chronogest海绵替换，再放置7天。取出海绵后，当天注射eCG，预计48小时后发情。在预计发情后的12小时和24小时，用冻融精液进行两次人工授精。每次放置装置或海绵时以及人工授精后40天，从颈静脉采集外周血。通过RIA测量血浆中P4和E2的浓度。通过糖蛋白测定监测人工授精的有效性，并通过出生的犊牛数量进行观察。根据妊娠相关糖蛋白（PAG）浓度，第一组确认有2例怀孕，第二组有5例怀孕。第一组观察到1头新生犊牛，第二组观察到5头。基于所获得的类固醇水平，两种同步化方法对母鹿均有效。尽管Chronogest海绵的形状比CIDR更好，因为CIDR不能完全放置在母鹿阴道内，可能导致细菌感染，并干扰正常的内分泌调节。此外，第二组的妊娠率和激素反应性更好。
