Laboratorio de Biología Molecular y Virología, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carr Federal Atlixco-Metepec, 74360, Metepec, Puebla, México.
Laboratorio de Biotecnología de Productos Naturales, Centro de Investigación Biomédica de Oriente, Instituto Mexicano del Seguro Social, Km 4.5 Carretera Federal Atlixco-Metepec, CP 74360, Metepec, Puebla, México.
BMC Complement Altern Med. 2018 Mar 16;18(1):95. doi: 10.1186/s12906-018-2163-3.
Urtica dioica, Taraxacum officinale, Calea integrifolia and Caesalpinia pulcherrima are widely used all over the world for treatment of different illnesses. In Mexico, these plants are traditionally used to alleviate or counteract rheumatism and inflammatory muscle diseases. In the present study we evaluated the activity of aqueous and methanolic extracts of these four plants, on the replication of dengue virus serotype 2 (DENV2).
Extraction process was carried out in a Soxtherm® system at 60, 85 and 120 °C; a chemical fractionation in silica gel chromatography was performed and compounds present in the active fractions were identified by HPLC-DAD-ESI/MSn. The cytotoxic concentration and the inhibitory effect of extracts or fractions on the DENV2 replication were analyzed in the BHK-21 cell line (plaque forming assay). The half maximal inhibitory concentration (IC) and the selectivity index (SI) were calculated for the extracts and fractions.
The methanolic extracts at 60 °C of T. officinale and U. dioica showed the higher inhibitory effects on DENV2 replication. After the chemical fractionation, the higher activity fraction was found for U. dioica and T. officinale, presenting IC values of 165.7 ± 3.85 and 126.1 ± 2.80 μg/ml, respectively; SI values were 5.59 and 6.01 for each fraction. The compounds present in T. officinale, were luteolin and caffeoylquinic acids derivatives and quercertin diclycosides. The compounds in the active fraction of U. dioica, were, chlorogenic acid, quercertin derivatives and flavonol glycosides (quercetin and kaempferol).
Two fractions from U. dioica and T. officinale methanolic extracts with anti-dengue activity were found. The compounds present in both fractions were identified, several recognized molecules have demonstrated activity against other viral species. Subsequent biological analysis of the molecules, alone or in combination, contained in the extracts will be carried out to develop therapeutics against DENV2.
荨麻、蒲公英、筋骨草和美丽异木棉在全世界范围内广泛用于治疗各种疾病。在墨西哥，这些植物传统上用于缓解或对抗风湿和炎症性肌肉疾病。在本研究中，我们评估了这四种植物的水提物和甲醇提取物对登革热病毒 2 型（DENV2）复制的活性。
采用 Soxtherm®系统在 60、85 和 120°C 下进行提取过程；在硅胶色谱上进行化学分级分离，并通过 HPLC-DAD-ESI/MSn 鉴定活性馏分中的化合物。在 BHK-21 细胞系（噬菌斑形成试验）中分析提取物或馏分对 DENV2 复制的细胞毒性浓度和抑制作用。计算提取物和馏分的半数最大抑制浓度（IC）和选择性指数（SI）。
蒲公英和荨麻的 60°C 甲醇提取物对 DENV2 复制表现出更高的抑制作用。经过化学分级分离，荨麻和蒲公英的活性馏分显示出更高的活性，其 IC 值分别为 165.7±3.85 和 126.1±2.80μg/ml；每个馏分的 SI 值分别为 5.59 和 6.01。蒲公英中的化合物为叶黄素和咖啡酰奎宁酸衍生物以及槲皮素二糖苷。荨麻活性馏分中的化合物为绿原酸、槲皮素衍生物和黄酮醇糖苷（槲皮素和山奈酚）。
从荨麻和蒲公英的甲醇提取物中发现了两种具有抗登革热活性的馏分。鉴定了两种馏分中存在的化合物，其中一些已被证明对其他病毒具有活性。随后将对提取物中所含的化合物单独或联合进行生物分析，以开发针对 DENV2 的治疗方法。
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