Menis Candela Florencia, Soria Elio Andrés, Moliva Melina Vanesa, Suárez Perrone Agostina, Reinoso Elina Beatríz, Giordano Walter, Sabini María Carola
Departament of Microbiology and Inmunology, Virology Area, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, Río Cuarto CP 5800, Argentina.
Instituto de Investigaciones en Ciencias de la Salud (INICSA), Consejo Nacional de Investigaciones Científicas y Técnicas, Boulevard de la Reforma y Enfermera Gordillo Gómez, Ciudad Universitaria, Córdoba Capital CP 5016, Argentina.
Plants (Basel). 2024 Oct 15;13(20):2881. doi: 10.3390/plants13202881.
Dengue is an emerging disease of high impact on human health. Plants are an important source of new antivirals and stands for its biological properties. The aim of this study was to evaluate the cytotoxicity and antiviral activity and elucidate the antiviral mechanism of ethanolic extracts from against dengue virus 2 (DENV-2). The skin or tegument ethanolic extract (TEEs) and seed ethanolic extract (SEEs) were obtained. Cytotoxicity was evaluated by MTT and Neutral Red Uptake (NRU). Antiviral activity was evaluated at different stages of the viral replication cycle by the lysis plaque reduction method. The 50% inhibitory concentration (IC) and selectivity index (SI) were determined. Antiviral activity was further determined by RT-qPCR. The CC values were 169 (NRU) and 65 (MTT) µg/mL for TEE. In addition, the CC values were >1400 (NRU) and 636 (MTT) µg/mL for SEE. The TEE demonstrated 99.9 ± 0.1% viral inhibition. The TEE presented an IC = 3.47 and SI of 48.7 (NRU) and 18.73 (MTT). Its mechanism of antiviral action is broad and it acts in the viral adsorption-penetration stage and inhibits the first steps of infection in the post-penetration stage. It is also capable of acting as virucidal and as prophylactic. Studies of RT-qPCR indicated that the TEE inhibited viral RNA synthesis. These findings suggest that the TEE from could be a promising antiviral candidate for treating DENV-2 infections.
登革热是一种对人类健康有重大影响的新兴疾病。植物是新型抗病毒药物的重要来源，并因其生物学特性而备受关注。本研究旨在评估[植物名称]乙醇提取物对登革热病毒2型（DENV-2）的细胞毒性和抗病毒活性，并阐明其抗病毒机制。制备了皮肤或外皮乙醇提取物（TEEs）和种子乙醇提取物（SEEs）。通过MTT法和中性红摄取法（NRU）评估细胞毒性。通过噬斑减少法在病毒复制周期的不同阶段评估抗病毒活性。测定了50%抑制浓度（IC）和选择性指数（SI）。通过RT-qPCR进一步确定抗病毒活性。TEE的CC值分别为169（NRU）和65（MTT）μg/mL。此外，SEE的CC值分别>1400（NRU）和636（MTT）μg/mL。TEE表现出99.9±0.1%的病毒抑制率。TEE的IC值为3.47，SI值为48.7（NRU）和18.73（MTT）。其抗病毒作用机制广泛，作用于病毒吸附-穿透阶段，并在穿透后阶段抑制感染的第一步。它还具有杀病毒和预防作用。RT-qPCR研究表明，TEE抑制病毒RNA合成。这些发现表明，[植物名称]的TEE可能是治疗DENV-2感染的有前途的抗病毒候选药物。
