College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Hum Genomics. 2018 Mar 16;12(1):15. doi: 10.1186/s40246-018-0146-6.
Previous studies demonstrated that massive induction of transcriptional readthrough generates downstream of gene-containing transcripts (DoGs) in cells under stress condition. Here, we analyzed TSS-seq (transcription start site sequencing) data from the DBTSS database. We investigated TSS tags at the end of gene for all pan-stress and untreated-cell DoGs, in comparison with expression-matched non-DoGs. We observed significantly more TSS tags at the end of pan-stress and untreated-cell DoG genes than non-DoG genes, even though their TSS tags in the promoter is the same. Importantly, the median value of TSS tags at gene end normalized to gene promoter is significantly higher than the median expression ratio of short DoG to host gene and of long DoG to host gene. Our results indicate that downstream overlapping long non-coding RNAs derived from the TSS at the gene end may be an important source of DoGs.
先前的研究表明，在应激条件下，大量转录通读会产生基因内含子转录本（DoGs）的下游产物。在这里，我们分析了 DBTSS 数据库中的 TSS-seq（转录起始位点测序）数据。我们比较了所有泛应激和未经处理细胞 DoGs 以及表达匹配的非 DoGs 的基因末端的 TSS 标签。与非 DoG 基因相比，即使它们的 TSS 标签在启动子中是相同的，泛应激和未经处理细胞 DoG 基因末端的 TSS 标签数量明显更多。重要的是，基因末端 TSS 标签与基因启动子归一化后的中位数显著高于短 DoG 与宿主基因以及长 DoG 与宿主基因的中位数表达比值。我们的结果表明，源自基因末端 TSS 的下游重叠长非编码 RNA 可能是 DoGs 的重要来源。
