Mitsuma T, Nogimori T, Sun D H, Chaya M
Exp Clin Endocrinol. 1987 Mar;89(1):55-60. doi: 10.1055/s-0029-1210627.
Effects of thyrotropin-releasing hormone (TRH) on the plasma beta-endorphin-like immunoreactivity (beta-Ep-LI) levels in rats have been studied. TRH (10 mg/kg) were injected i.v., and the animals were serially decapitated. The plasma beta-Ep-LI levels were measured by radioimmunoassay. Effects of TRH on beta-Ep-LI release from the anterior pituitary were also investigated by means of an in vitro experiment. The beta-Ep-LI content in the hypothalamus, pituitary gland or adrenal gland did not change significantly after TRH injection. The plasma beta-Ep-LI levels decreased significantly in a dose-related manner with a nadir at 40 min after the injection. In the hypophysectomized rats, the plasma beta-Ep-LI levels were lower than that of the control, and did not change significantly after TRH injection. beta-Ep-LI release from anterior pituitary in vitro significantly decreased after the addition of TRH to medium. These findings suggest that TRH may act on the pituitary gland to reduce plasma beta-Ep-LI levels in rats.
已经研究了促甲状腺激素释放激素（TRH）对大鼠血浆β-内啡肽样免疫反应性（β-Ep-LI）水平的影响。静脉注射TRH（10毫克/千克），然后对动物进行连续断头处死。通过放射免疫测定法测量血浆β-Ep-LI水平。还通过体外实验研究了TRH对垂体前叶β-Ep-LI释放的影响。注射TRH后，下丘脑、垂体或肾上腺中的β-Ep-LI含量没有明显变化。注射后40分钟时，血浆β-Ep-LI水平以剂量相关的方式显著下降，达到最低点。在垂体切除的大鼠中，血浆β-Ep-LI水平低于对照组，注射TRH后没有明显变化。在培养基中添加TRH后，垂体前叶体外β-Ep-LI释放显著减少。这些发现表明，TRH可能作用于垂体，以降低大鼠血浆β-Ep-LI水平。
