泛酸钙，一种γ-氨基丁酸（GABA）激动剂，可使大鼠血浆中β-内啡肽样免疫反应性水平升高。

Calcium hopantenate, a GABA agonist, induces elevation of plasma beta-endorphin-like immunoreactivity levels in rats.

作者信息

Mitsuma T, Sun D H, Nogimori T, Chaya M, Ohtake K, Hirooka Y

出版信息

Exp Clin Endocrinol. 1987 Apr;89(2):181-7.

Abstract

The effects of calcium hopantenate (HOPA) on plasma beta-endorphin-like immunoreactivity (beta-En-LI) levels in rats have been studied. HOPA (1.5 g/kg) was injected ip, and the animals were serially decapitated. The plasma beta-En-LI levels were measured by radioimmunoassay. Effects of HOPA on beta-En-LI release from the anterior pituitary were also investigated by means of an in vitro experiment. The beta-En-LI content in the hypothalamus and pituitary gland did not change significantly after HOPA injection. The plasma beta-En-LI levels increased significantly in a dose-related manner with a zenith at 30 min after HOPA injection. Effects of HOPA on plasma beta-En-LI levels were prevented with the pretreatment of bicuculline. The beta-En-LI release from the anterior pituitary was enhanced with the addition of HOPA to the medium, and HOPA's effects were blocked with an addition of bicuculline to the medium. These findings suggest that HOPA acts on the anterior pituitary to stimulate beta-En-LI release, and its effects are mediated via GABA receptor.

摘要

已对泛癸利酮（HOPA）对大鼠血浆β-内啡肽样免疫反应性（β-En-LI）水平的影响进行了研究。腹腔注射HOPA（1.5 g/kg），然后将动物依次断头处死。通过放射免疫测定法测量血浆β-En-LI水平。还通过体外实验研究了HOPA对垂体前叶β-En-LI释放的影响。注射HOPA后，下丘脑和垂体中的β-En-LI含量没有明显变化。血浆β-En-LI水平在注射HOPA后30分钟呈剂量相关方式显著升高，并达到峰值。用荷包牡丹碱预处理可阻止HOPA对血浆β-En-LI水平的影响。向培养基中添加HOPA可增强垂体前叶的β-En-LI释放，而向培养基中添加荷包牡丹碱可阻断HOPA的作用。这些发现表明，HOPA作用于垂体前叶以刺激β-En-LI释放，其作用是通过GABA受体介导的。