接种镰刀菌后厚皮甜瓜果实中 NEO 的检测及其采后臭氧处理的控制。

Detection of NEO in muskmelon fruits inoculated with Fusarium sulphureum and its control by postharvest ozone treatment.

机构信息

College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.

College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.

出版信息

Food Chem. 2018 Jul 15;254:193-200. doi: 10.1016/j.foodchem.2018.01.149. Epub 2018 Jan 31.

DOI:10.1016/j.foodchem.2018.01.149

PMID:29548441

Abstract

Fusarium rot of muskmelon, caused by Fusarium spp., is one of the most important postharvest decays, that not only causes economic losses but leads to trichothecenes contamination. A rapid and sensitive method was developed for neosolaniol (NEO) analysis in muskmelon inoculated with F. sulphureum, utilizing acetonitrile/water (84:16, v/v) extraction and PriboFast M270 columns purification and UPLC-MS/MS detection. Method validation was evaluated by linearity (R ≥ 0.9990), recovery (88.1-136.9%), precision (RSD ≤ 3.97%) and sensitivity (LOD, 0.5 μg/kg; LOQ, 1.5 μg/kg). The effect of ozone treatment on Fusarium rot development and NEO accumulation in inoculated muskmelon was also evaluated. The results showed that UPLC-MS/MS method was suitable for analyzing NEO in inoculated muskmelon, and 1.10 mg/l ozone treatment for 120 min significantly controlled Fusarium rot development and NEO accumulation in fruits after 5, 8 and 11 days. In vivo tests showed that ozone at 1.10 mg/l effectively degraded NEO in acetonitrile.

摘要

镰刀菌腐烂病是由镰刀菌引起的，是甜瓜采后最重要的腐烂病之一，不仅造成经济损失，还会导致呕吐毒素污染。本研究建立了利用乙腈/水（84:16，v/v）提取和 PriboFast M270 柱净化，结合 UPLC-MS/MS 检测，用于检测接种 F. sulphureum 的甜瓜中 neo 索拉醇（NEO）的快速灵敏方法。通过线性（R ≥ 0.9990）、回收率（88.1-136.9%）、精密度（RSD ≤ 3.97%）和灵敏度（LOD，0.5 μg/kg；LOQ，1.5 μg/kg）对方法进行验证。还评估了臭氧处理对接种甜瓜镰刀菌腐烂病发展和 NEO 积累的影响。结果表明，UPLC-MS/MS 方法适用于接种甜瓜中 NEO 的分析，1.10 mg/l 臭氧处理 120 min 可显著控制接种后 5、8 和 11 天果实中镰刀菌腐烂病的发展和 NEO 的积累。体内试验表明，1.10 mg/l 的臭氧可有效降解乙腈中的 NEO。