Xi Jihui, Liu Qili, Zhang Qingru, Liu Zhiguang, Xue Huali, Feng Yuqin
College of Science, Gansu Agricultural University, Lanzhou 730070, China.
College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China.
J Fungi (Basel). 2025 May 23;11(6):402. doi: 10.3390/jof11060402.
is a homologous plant of medicine and food with a variety of health benefits. However, our previous study showed that blue mold, caused by , is the most important postharvest disease of fresh during storage. Ozone, as a strong oxidizing agent, can effectively control the occurrence of postharvest diseases in fruits and vegetables. Nevertheless, there are few research studies on the effect of ozone-treated fresh Chinese traditional medicine. In this study, we investigated the effect of ozone gas on the postharvest blue mold development, mycotoxin accumulation, and main active component contents in fresh infected by , and analyzed the possible action mechanism on ROS metabolism. The result indicates that ozone application significantly inhibited the development of postharvest blue mold caused by infection, reduced the disease incidence, disease index, and weight loss rate, maintained the main active ingredients in fresh by activating ROS metabolism, enhanced the antioxidant enzymatic activity, thus avoiding oxidative damage caused by excessive ROS accumulation, and maintained the integrity of the cell membrane, ultimately controlling the occurrence of blue mold of . Moreover, ozone treatment also maintained the contents of the main active ingredients in before 14 d during infection. In addition, the amount of active ingredients of astragaloside I, calycosin-7-glucoside, and ononin in the ozone-treated group was higher than that in the control group during the storage period. We speculate that, under the action of ozone, astragaloside IV was converted into astragaloside II by oxidative modification and astragaloside II was further oxidized to astragaloside I, resulting in the accumulation of astragaloside I. Similarity, the hydrogen atoms (-H) on the benzene ring in formononetin were oxidized to phenolic hydroxyl groups (-OH) to generate calycosin, which was further converted into calycosin-7-glucoside, resulting in calycosin-7-glucoside accumulation. This study will provide the theoretical basis for ozone commercial application to control the occurrence of postharvest diseases of .
是一种药食同源植物，具有多种健康益处。然而，我们之前的研究表明，由[具体病菌名称未给出]引起的青霉病是新鲜[植物名称未给出]贮藏期间最重要的采后病害。臭氧作为一种强氧化剂，能有效控制果蔬采后病害的发生。然而，关于臭氧处理新鲜中药材的效果的研究较少。在本研究中，我们研究了臭氧气体对受[具体病菌名称未给出]感染的新鲜[植物名称未给出]采后青霉病发展、霉菌毒素积累和主要活性成分含量的影响，并分析了其对活性氧代谢的可能作用机制。结果表明，臭氧处理显著抑制了由[具体病菌名称未给出]感染引起的采后青霉病发展，降低了发病率、病情指数和失重率，通过激活活性氧代谢维持了新鲜[植物名称未给出]中的主要活性成分，增强了抗氧化酶活性，从而避免了因活性氧过度积累引起的氧化损伤，维持了细胞膜的完整性，最终控制了[植物名称未给出]青霉病的发生。此外，臭氧处理在[具体病菌名称未给出]感染期间14天之前也维持了[植物名称未给出]中主要活性成分的含量。另外，在贮藏期间，臭氧处理组中黄芪甲苷I、毛蕊异黄酮葡萄糖苷和芒柄花苷的活性成分含量高于对照组。我们推测，在臭氧作用下，黄芪甲苷IV通过氧化修饰转化为黄芪甲苷II，黄芪甲苷II进一步氧化为黄芪甲苷I，导致黄芪甲苷I积累。类似地，刺芒柄花素苯环上的氢原子(-H)被氧化为酚羟基(-OH)生成毛蕊异黄酮，毛蕊异黄酮进一步转化为毛蕊异黄酮葡萄糖苷，导致毛蕊异黄酮葡萄糖苷积累。本研究将为臭氧商业应用控制[植物名称未给出]采后病害的发生提供理论依据。
