Division of Regulatory Toxicology, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, M.P., India.
Jamia Hamdard, New Delhi, India.
Chem Biol Interact. 2018 Apr 25;286:78-87. doi: 10.1016/j.cbi.2018.03.006. Epub 2018 Mar 13.
Chelation therapy is the mainstream treatment for heavy metal poisoning. Apart from this, therapy using antioxidant/herbal extracts are the other strategies now commonly being tried for the treatment. We have previously reported individual beneficial efficacy of nanoparticle mediated administration of an antioxidant like 'curcumin' and an arsenic chelator 'monoisoamyl 2,3-dimercaptosuccinic acid (MiADMSA)' for the treatment of arsenic toxicity compared to bulk drugs. The present paper investigates our hypothesis that a combination drug delivery therapy employing two nanosystems, a chelator and a strong antioxidant, may produce more pronounced therapeutic effects compared to individual effects in the treatment of arsenic toxicity. An in-vivo study was conducted wherein arsenic as sodium arsenite (100 ppm) was administered in drinking water for 5 months to Swiss albino mice. This was followed by a treatment protocol comprising of curcumin encapsulated chitosan nanoparticles (nano-curcumin, 15 mg/kg, orally for 1 month) either alone or in combination with MiADMSA encapsulated polymeric nanoparticles (nano-MiADMSA, 50 mg/kg for last 5 days) to evaluate the therapeutic potential of the combination treatment. Our results demonstrated that co-treatment with nano-curcumin and nano-MiADMSA provided beneficial effects in a synergistic way on the adverse changes in oxidative stress parameters and metal status induced by arsenic.
螯合疗法是重金属中毒的主流治疗方法。除此之外，现在还普遍尝试使用抗氧化剂/草药提取物进行治疗。我们之前已经报道过，与原料药相比，纳米颗粒介导的抗氧化剂“姜黄素”和砷螯合剂“单异戊基 2,3-二巯基丁二酸（MiADMSA）”的个体治疗对砷毒性具有个体有益的疗效。本文研究了我们的假设，即联合药物输送疗法，采用两种纳米系统，一种是螯合剂，另一种是强抗氧化剂，可能会产生比单独治疗砷毒性更明显的治疗效果。进行了一项体内研究，其中在饮用水中以亚砷酸钠（100ppm）给瑞士白化病小鼠喂食 5 个月。随后，采用姜黄素包封壳聚糖纳米颗粒（纳米姜黄素，15mg/kg，口服，持续 1 个月）单独或与 MiADMSA 包封聚合物纳米颗粒（纳米-MiADMSA，50mg/kg，最后 5 天）联合治疗方案，以评估联合治疗的治疗潜力。我们的结果表明，纳米姜黄素和纳米-MiADMSA 的联合治疗以协同方式对砷诱导的氧化应激参数和金属状态的不利变化提供了有益的效果。
