Seok Hye-Yeon, Nguyen Linh Vu, Park Hee-Yeon, Tarte Vaishali N, Ha Jimin, Lee Sun-Young, Moon Yong-Hwan
Department of Integrated Biological Science, Pusan National University, Busan, 46241, Republic of Korea.
Department of Integrated Biological Science, Pusan National University, Busan, 46241, Republic of Korea.
Biochem Biophys Res Commun. 2018 Apr 15;498(4):954-959. doi: 10.1016/j.bbrc.2018.03.088. Epub 2018 Mar 17.
Functional studies of CCCH-type zinc finger proteins in abiotic stress responses have largely focused on tandem CCCH-type zinc finger (TZF) genes, whereas the study of functional roles of non-TZF genes in abiotic stress responses has largely been neglected. Here, we investigated the functional roles of AtC3H17, a non-TZF gene of Arabidopsis, in salt stress responses. AtC3H17 expression significantly increased under NaCl, mannitol, and ABA treatments. AtC3H17-overexpressing transgenic plants (OXs) were more tolerant under NaCl and MV treatment conditions than the wild type (WT). atc3h17 mutants were more sensitive under NaCl and MV treatment conditions compared with the WT. The transcription of the salt stress-responsive genes in ABA-dependent pathway, such as RAB18, COR15A, and RD22, was significantly higher in AtC3H17 OXs than in WT both under NaCl-free condition and after NaCl treatment. Our results demonstrate that AtC3H17 functions as a positive regulator in salt stress response, via the up-regulation of ABA-dependent salt stress-response pathway.
CCCH型锌指蛋白在非生物胁迫响应中的功能研究主要集中在串联CCCH型锌指（TZF）基因上，而非TZF基因在非生物胁迫响应中的功能作用研究在很大程度上被忽视了。在此，我们研究了拟南芥的一个非TZF基因AtC3H17在盐胁迫响应中的功能作用。在NaCl、甘露醇和ABA处理下，AtC3H17的表达显著增加。在NaCl和MV处理条件下，过表达AtC3H17的转基因植株（OXs）比野生型（WT）更耐胁迫。与WT相比，atc3h17突变体在NaCl和MV处理条件下更敏感。在无NaCl条件下和NaCl处理后，ABA依赖途径中盐胁迫响应基因如RAB18、COR15A和RD22在AtC3H17 OXs中的转录显著高于WT。我们的结果表明，AtC3H17通过上调ABA依赖的盐胁迫响应途径，在盐胁迫响应中起正调控作用。
