School of Forestry, Northeast Forestry University, Harbin 150040, PR China; Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Gene. 2018 Jun 15;659:100-108. doi: 10.1016/j.gene.2018.03.030. Epub 2018 Mar 14.
Northern corn leaf blight (NCLB), caused by the hemibiotrophic fungal pathogen Setosphaeria turcica, is one of the major foliar diseases of maize. The use of resistant cultivars is the most effective, economical, and environmentally friendly means to control NCLB. At present, the molecular mechanisms of maize resistance to S. turcica is not clear. Elucidating the molecular resistance mechanisms of maize response to S. turcica would aid breeding for a maize variety with fungal tolerance. In this study, maize leaves before and after infection with S. turcica were sequenced by RNA-seq, and 5903 differentially expressed genes (DEGs) were screened. Among them, 950 and 2245 genes were up-regulated 12 h and 60 h (samples H12 and H60, respectively) after infection, 752 and 1956 genes were down-regulated in H12 and H60, respectively. The GO and KEGG enrichment analysis of the DEGs showed that the GO and Pathway with the most annotation sequences were closely related to plant resistance. The expression of eight randomly selected DEGs was analyzed using qRT-PCR, and expression was consistent with the RNA-seq data. The expression patterns of four categories of genes were analyzed namely, genes involved in plant and pathogen interactions, transcription factors related to plant stress-tolerance, genes related to plant hormones and plant antioxidant. Many resistant signaling pathways were initiated such as the MAPK signal transduction pathway and the expression of multiple antioxidant-related genes [Peroxidase (POD), Catalase (CAT), Glutathione-S-transferase (GST) and Superoxide Dismutase (SOD)] following S. turcica infection. Many disease resistance signal transduction pathways and defense response pathways were induced following maize infection by S. turcica, suggesting a multiple gene network system. To the best of our knowledge, this is the first time that RNA-seq technology has been used to perform transcription analysis of maize in response to S. turcica stress. Taken together, these data provide novel and valuable information that will help understand the resistance mechanism in maize against S. turcica and locate candidate genes related to maize resistance against S. turcica.
北方玉米叶枯病（NCLB）是由半生物型真菌病原体玉蜀黍节壶菌引起的一种主要玉米叶部病害。使用抗性品种是控制 NCLB 的最有效、经济和环保的方法。目前，玉米对 S. turcica 的分子抗性机制尚不清楚。阐明玉米对 S. turcica 响应的分子抗性机制将有助于培育对真菌具有耐受性的玉米品种。在这项研究中，通过 RNA-seq 对感染 S. turcica 前后的玉米叶片进行了测序，筛选出 5903 个差异表达基因（DEGs）。其中，感染后 12 小时（样本 H12）和 60 小时（样本 H60）分别有 950 和 2245 个基因上调，分别有 752 和 1956 个基因下调。DEGs 的 GO 和 KEGG 富集分析表明，GO 和通路注释序列最多的与植物抗性密切相关。用 qRT-PCR 分析了 8 个随机选择的 DEGs 的表达情况，结果与 RNA-seq 数据一致。对四类基因的表达模式进行了分析，即涉及植物与病原体相互作用的基因、与植物抗逆性相关的转录因子、与植物激素和植物抗氧化剂相关的基因。玉蜀黍节壶菌感染后，启动了许多抗性信号通路，如 MAPK 信号转导通路和多个抗氧化相关基因（过氧化物酶（POD）、过氧化氢酶（CAT）、谷胱甘肽 S-转移酶（GST）和超氧化物歧化酶（SOD））的表达。玉米感染 S. turcica 后，诱导了许多抗病信号转导途径和防御反应途径，表明存在一个多基因网络系统。据我们所知，这是首次使用 RNA-seq 技术对玉米响应 S. turcica 胁迫进行转录分析。综上所述，这些数据提供了新的有价值的信息，有助于了解玉米对 S. turcica 的抗性机制，并定位与玉米对 S. turcica 抗性相关的候选基因。
