Scholl Aaron, Yang Yuyang, McBride Patrick, Irwin Kelly, Jiang Lan
Department of Biological Sciences, Oakland University, USA.
Department of Biological Sciences, Oakland University, USA.
Gene Expr Patterns. 2018 Jun;28:87-94. doi: 10.1016/j.gep.2018.03.001. Epub 2018 Mar 14.
The Drosophila trachea is a premier genetic system to investigate the fundamental mechanisms of tubular organ formation. Development of the trachea consists of the well understood early branch specification and migration processes, and the less clear later branch maturation process including the apical membrane expansion, cytoskeleton rearrangement, luminal matrix clearance, and air-filling. We identified seven members of the Osiris (Osi) gene family with obvious tracheal expression in Drosophila. In addition, HA-tagged Osi proteins are highly concentrated in vesicle-like structures at and near the apical membrane. Osi proteins are predicted to contain endocytic signals and transmembrane domains. The localization of Osi proteins is consistent with these predictions. Interestingly, the Drosophila tracheal tube maturation process also occurs at the apical membrane. Taken together, the localization of Osi proteins suggest that these proteins are likely involved in tube maturation through vesicular trafficking or interacting with other apical membrane proteins.
果蝇气管是研究管状器官形成基本机制的首要遗传系统。气管的发育包括人们熟知的早期分支特化和迁移过程，以及不太清楚的后期分支成熟过程，包括顶端膜扩张、细胞骨架重排、管腔基质清除和空气填充。我们在果蝇中鉴定出七个在气管中有明显表达的奥西里斯（Osi）基因家族成员。此外，带有HA标签的Osi蛋白高度集中在顶端膜及其附近的囊泡样结构中。Osi蛋白预计含有内吞信号和跨膜结构域。Osi蛋白的定位与这些预测一致。有趣的是，果蝇气管管成熟过程也发生在顶端膜。综上所述，Osi蛋白的定位表明这些蛋白可能通过囊泡运输或与其他顶端膜蛋白相互作用参与管成熟。
