Kusuki Mari, Osawa Kayo, Arikawa Kentaro, Tamura Miho, Shigemura Katsumi, Shirakawa Toshiro, Nakamura Tatsuya, Nakamachi Yuji, Fujisawa Masato, Saegusa Jun, Tokimatsu Issei
Department of Clinical Laboratory, Kobe University Hospital, Kobe, Japan; Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan.
Department of Infection Control and Prevention, Kobe University Hospital, Kobe, Japan; Department of Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan.
Diagn Microbiol Infect Dis. 2018 Jul;91(3):256-259. doi: 10.1016/j.diagmicrobio.2018.02.008. Epub 2018 Feb 18.
Mycobacterium abscessus complex, including three subspecies-M. abscessus, M. massiliense, and M. bolletii-is resistant to a variety of antibiotics so limited treatment options are available. The susceptibility of these subspecies to antimicrobial agents depends in particular on the erm(41) sequevar and rrl mutations in the 23S rRNA, which are potentially related to clarithromycin (CLR) resistance. The purpose of this study was to carry out identification and molecular characterization of these subspecies based on variable number of tandem repeats (VNTR) analysis. Twenty-four M. abscessus complex strains were identified as M. abscessus and M. massiliense and these subspecies could be discriminated between based on their resistance to CLR, as determined by truncation or mutation of erm(41) or mutation of rrl, as illustrated by their VNTR patterns. In conclusion, we confirmed that the CLR susceptibility profiles could be differentiated according to the subspecies of M. abscessus complex strains by their VNTR patterns.
脓肿分枝杆菌复合体，包括三个亚种——脓肿分枝杆菌、马西利亚分枝杆菌和博列蒂分枝杆菌——对多种抗生素耐药，因此可用的治疗选择有限。这些亚种对抗菌药物的敏感性尤其取决于23S rRNA中的erm(41)序列变异和rrl突变，这可能与克拉霉素（CLR）耐药性有关。本研究的目的是基于可变数目串联重复序列（VNTR）分析对这些亚种进行鉴定和分子特征分析。24株脓肿分枝杆菌复合体菌株被鉴定为脓肿分枝杆菌和马西利亚分枝杆菌，根据它们对CLR的耐药性，通过erm(41)的截断或突变或rrl的突变来区分这些亚种，这通过它们的VNTR模式得以体现。总之，我们证实，通过VNTR模式可以根据脓肿分枝杆菌复合体菌株的亚种区分CLR敏感性谱。
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