利奈唑胺耐药临床分离株的分子分析。
Molecular Analysis of Linezolid-Resistant Clinical Isolates of .
机构信息
Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, China.
Tongji University School of Medicine, Shanghai, China.
出版信息
Antimicrob Agents Chemother. 2019 Jan 29;63(2). doi: 10.1128/AAC.01842-18. Print 2019 Feb.
A total of 194 isolates were collected from patients, and the whole genomes were sequenced. Eighty-five (43.8%) isolates showed linezolid (LZD) resistance. Only 8.2% of resistant isolates harbored 23S rRNA mutations. Quantitative real-time PCR (qRT-PCR) revealed higher transcriptional levels of efflux pumps and in LZD-resistant isolates. Genome comparative analysis identified several new LZD resistance-associated genes. This study highlights the role of efflux pumps in LZD-resistant and proposes potential target genes for further studies.
共从患者中收集了 194 株分离株，并对其全基因组进行了测序。85 株（43.8%）分离株对利奈唑胺（LZD）表现出耐药性。仅有 8.2%的耐药分离株携带 23S rRNA 突变。实时荧光定量 PCR（qRT-PCR）显示，LZD 耐药分离株中泵的转录水平更高。基因组比较分析确定了几个新的与 LZD 耐药相关的基因。本研究强调了泵在 LZD 耐药中的作用，并提出了进一步研究的潜在靶基因。
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