Akdeniz University, Department of Agricultural Biotechnology, Antalya, Turkey.
Duzce University, Department of Crop and Animal Production, Cilimli Vocational School, Cilimli, Duzce, Turkey.
Genomics. 2019 Jul;111(4):579-589. doi: 10.1016/j.ygeno.2018.03.013. Epub 2018 Mar 14.
ATP sulfurylase (ATPS, EC: 2.7.7.4) is a crucial enzyme for sulfate assimilation pathway in both plastids and cytosol in plants. In this study, genome-wide and comparative analyses of ATPSs in 11 higher plant species, including sequence and structural analyses have been performed. Expression of ATPS genes in sorghum under cadmium (Cd) and salinity (NaCl) stresses were also investigated to provide a model experimental data for the regulation of ATPS genes under stress conditions. Thirty-one ATPS genes from 11 plant species were found. It showed that ATPSs from different species have high sequence divergences, which cause structural differences among them. Phylogenetic analysis has shown that there are two major types of ATPSs evolved in dicots while monocots were evolved to have one type of ATPs. Finally, expression analysis of ATPS genes revealed tissue and stress dependent expression pattern, which indicates expressions of ATPS genes are tightly regulated.
三磷酸腺苷硫酸化酶（ATPS，EC：2.7.7.4）是植物质体和胞质溶胶中硫酸盐同化途径的关键酶。在这项研究中，对 11 种高等植物中的 ATPS 进行了全基因组和比较分析，包括序列和结构分析。还研究了高粱在镉（Cd）和盐（NaCl）胁迫下 ATPS 基因的表达，为胁迫条件下 ATPS 基因的调控提供了模型实验数据。从 11 种植物中发现了 31 个 ATPS 基因。结果表明，不同物种的 ATPS 具有很高的序列差异，导致它们之间存在结构差异。系统发育分析表明，双子叶植物中有两种主要类型的 ATPS 进化而来，而单子叶植物则进化为一种 ATPs。最后，ATPS 基因的表达分析显示出组织和胁迫依赖性表达模式，表明 ATPS 基因的表达受到严格调控。
