Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, 350007, China.
Provincial University Key Laboratory of Cellular Stress Response and Metabolic Regulation, College of Life Sciences, Fujian Normal University, Fuzhou, 350117, China.
Biomech Model Mechanobiol. 2018 Aug;17(4):1209-1215. doi: 10.1007/s10237-018-1012-0. Epub 2018 Mar 17.
Mitosis is an important physiological event accompanying with dramatic changes of cellar biophysical properties. Failure of mitosis results in cell death or chromosome aneuploidy. In this study, we used atomic force microscopy to probe and compare the biophysical properties of tumor cells at different stages during mitosis. The rounding forces of MCF-7 cells oscillated during mitosis. At anaphase, the average elasticity of cells was higher than that at other phases. Cholesterol depletion with M[Formula: see text]CD led to an increase in the average elasticity, whereas the average roughness of membrane surface decreased at the absence of cholesterol. Our study indicated that the distribution of actin filaments could affect the biophysical properties of tumor cells and cellular morphology during mitosis. Furthermore, the biophysical properties of tumor cells were also regulated by membrane cholesterol during mitosis. This work provides a new detection approach for monitoring tumor cell development at single cell level.
有丝分裂是一个伴随着细胞生物物理性质剧烈变化的重要生理事件。有丝分裂失败会导致细胞死亡或染色体非整倍体。在这项研究中，我们使用原子力显微镜探测并比较了处于有丝分裂不同阶段的 MCF-7 肿瘤细胞的生物物理性质。在有丝分裂过程中，MCF-7 细胞的圆化力呈现出震荡变化。在后期，细胞的平均弹性高于其他阶段。用 MβCD 耗竭胆固醇会导致平均弹性增加，而在没有胆固醇的情况下，膜表面的平均粗糙度会降低。我们的研究表明，肌动蛋白丝的分布会影响肿瘤细胞在有丝分裂过程中的生物物理性质和细胞形态。此外，肿瘤细胞的生物物理性质也受到有丝分裂过程中膜胆固醇的调节。这项工作为在单细胞水平监测肿瘤细胞发育提供了一种新的检测方法。
