秋皇家葡萄和里比埃葡萄汁提取物降低结肠癌细胞的活力和转移潜力。
Autumn Royal and Ribier Grape Juice Extracts Reduced Viability and Metastatic Potential of Colon Cancer Cells.
作者信息
Valenzuela Manuel, Bastias Lorena, Montenegro Iván, Werner Enrique, Madrid Alejandro, Godoy Patricio, Párraga Mario, Villena Joan
机构信息
Facultad de Ciencias de la Salud, Universidad Central de Chile, 8330507 Santiago, Chile.
Laboratorio de Comunicaciones Celulares, Centro de Estudios Moleculares de la Célula (CEMC), Program of Cell and Molecular Biology, Institute of Biomedical Sciences (ICBM), School of Medicine, Universidad de Chile, 8380453 Santiago, Chile.
出版信息
Evid Based Complement Alternat Med. 2018 Jan 14;2018:2517080. doi: 10.1155/2018/2517080. eCollection 2018.
Antioxidants are known to be beneficial to health. This paper evaluates the potential chemopreventive and anticancer properties of phenolic compounds present in grape juice extracts (GJE) from Autumn Royal and Ribier varieties. The effects of these GJE on viability (SRB day assay) and metastatic potential (migration and invasion parameters) of colon cancer cell lines HT-29 and SW-480 were evaluated. The effects of GJE on two matrix metalloproteinase gene expressions (MMP2 and MMP9) were also evaluated via qRT-PCR. In the former, GJE reduced cell viability in both cell lines in a dose-dependent manner. GJE treatment also reduced cell migration and invasion. Moreover, MMP-2 and MMP-9 gene expression diminished depending on extract and on cell type. . These results provide novel information concerning anticancer properties of selected GJE by revealing selective cytotoxicity and the ability to reduce invasiveness of colon cancer cells.
已知抗氧化剂对健康有益。本文评估了秋皇家和里比埃品种葡萄汁提取物（GJE）中酚类化合物潜在的化学预防和抗癌特性。评估了这些GJE对结肠癌细胞系HT - 29和SW - 480的活力（SRB法）和转移潜能（迁移和侵袭参数）的影响。还通过qRT - PCR评估了GJE对两种基质金属蛋白酶基因表达（MMP2和MMP9）的影响。在前者中，GJE以剂量依赖的方式降低了两种细胞系中的细胞活力。GJE处理还减少了细胞迁移和侵袭。此外，MMP - 2和MMP - 9基因表达根据提取物和细胞类型而降低。这些结果通过揭示选择性细胞毒性和降低结肠癌细胞侵袭性的能力，提供了有关所选GJE抗癌特性的新信息。
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