Zhou Ling, Wang Min, Guo Chongyong, Zhu Ying, Yu Hua, Zhang Lu, Yu Pei
Department of Surgery, Branch of Shanghai First People's Hospital, Shanghai 200081, P.R. China.
Department of Pathology, Branch of Shanghai First People's Hospital, Shanghai 200081, P.R. China.
Oncol Lett. 2018 Apr;15(4):4859-4866. doi: 10.3892/ol.2018.7965. Epub 2018 Feb 6.
Over the past three decades, numerous patients with breast cancer succumbed to cancer metastasis and recurrence, while, the exact mechanisms underlying this malignancy, and the potential biomarkers for prognosis prediction remain elusive. It was previously demonstrated that phosphorylated RAC-α serine/threonine-protein kinase (pAkt) and Beclin 1 was associated with cancer metastasis, and recurrence. Thus far, the expression patterns of pAkt and Beclin 1 in breast cancer tissues, and their associations with the prognosis of invasive ductal breast cancer remain inconclusive, which may be due to various factors, including ethnicity and pathological types. In the present study, a total of 90 Chinese female patients with invasive ductal breast cancer between June 1999 and August 2002 were enrolled at Shanghai First People's Hospital (Shanghai, China). The patients were followed up from 5 months to 13.5 years for survival analysis. The expressional levels of pAkt and Beclin 1 in invasive ductal breast cancer tissues, and the normal paracancerous tissues were measured by immunohistochemistry. Associations with prognosis following surgery were further evaluated using Cox regression analysis. In 90 invasive ductal breast cancer samples, pAkt was detected in 17 (18.9%) samples and Beclin 1 in 33 (36.7%) samples, but both were not detected in any of the paracancerous samples. Survival analysis revealed that pAkt expression carried a tendency to predict a shorter disease-free survival (DFS) in patients with invasive ductal breast cancer. Additionally, Beclin 1 expression was not significantly associated with survival. Furthermore, univariate Cox regression analysis demonstrated that pAkt expression was negatively associated with DFS and overall survival. Multivariate Cox regression analysis indicated that pAkt expression was an independent risk factor associated with poor prognosis in patients with invasive ductal breast cancer (all P<0.05). pAkt may be used as a potential prognostic biomarker in Chinese women with invasive ductal breast cancer.
在过去三十年中，众多乳腺癌患者死于癌症转移和复发，然而，这种恶性肿瘤背后的确切机制以及用于预后预测的潜在生物标志物仍然难以捉摸。先前的研究表明，磷酸化的RAC-α丝氨酸/苏氨酸蛋白激酶（pAkt）和Beclin 1与癌症转移和复发有关。迄今为止，pAkt和Beclin 1在乳腺癌组织中的表达模式及其与浸润性导管癌预后的关联尚无定论，这可能是由于多种因素，包括种族和病理类型。在本研究中，1999年6月至2002年8月期间，共有90名中国女性浸润性导管癌患者在上海第一人民医院（中国上海）入组。对患者进行了5个月至13.5年的随访以进行生存分析。通过免疫组织化学测量浸润性导管癌组织和正常癌旁组织中pAkt和Beclin 1的表达水平。使用Cox回归分析进一步评估手术后与预后的关联。在90个浸润性导管癌样本中，17个（18.9%）样本检测到pAkt，33个（36.7%）样本检测到Beclin 1，但在任何癌旁样本中均未检测到两者。生存分析显示，pAkt表达倾向于预测浸润性导管癌患者较短的无病生存期（DFS）。此外，Beclin 1表达与生存无显著关联。此外，单因素Cox回归分析表明，pAkt表达与DFS和总生存期呈负相关。多因素Cox回归分析表明，pAkt表达是浸润性导管癌患者预后不良的独立危险因素（所有P<0.05）。pAkt可能作为中国浸润性导管癌女性患者潜在的预后生物标志物。
