Department of Maternal and Child Health Care, School of Public Health, Shandong University, Jinan, 250012, China.
Gynecol Oncol. 2012 Jul;126(1):109-17. doi: 10.1016/j.ygyno.2012.03.051. Epub 2012 Apr 6.
High mobility group box l (HMGB1), a nuclear and extracellular protein, is implicated in some physiologic and pathologic conditions. In this study, we investigated the expression and function of HMGB1 in ovarian cancer.
cDNA microarray analysis was performed to compare gene expression profiles of the highly invasive and the low invasive subclones derived from the SKOV3 human ovarian cancer cell line. Immunohistochemistry (IHC) staining was performed to investigate HMGB1 expression in a total of 100 ovarian tissue specimens. In functional assays, effects of HMGB1 knockdown on the biological behavior of ovarian cancer cells were investigated.
HMGB1 was overexpressed in the highly invasive subclone compared with the low invasive subclone. High HMGB1 expression was associated with poor clinicopathologic features. Knockdown of HMGB1 expression significantly suppressed ovarian cancer cell proliferation accompanied by decreased cyclin D1 and PCNA expression, and inhibited cell migration and invasion accompanied by decreased MMP2 and MMP9 activities.
HMGB1 is a newly identified gene overexpressed in ovarian cancer and associated with poor clinicopathologic features. HMGB1 may serve as a new biomarker and a therapeutic target for ovarian cancer in the future.
高迁移率族蛋白 B1（HMGB1）是一种核蛋白和细胞外蛋白，与一些生理和病理状况有关。在这项研究中，我们研究了 HMGB1 在卵巢癌中的表达和功能。
通过 cDNA 微阵列分析比较了源自 SKOV3 人卵巢癌细胞系的高侵袭性和低侵袭性亚克隆的基因表达谱。免疫组织化学（IHC）染色检测了总共 100 个卵巢组织标本中 HMGB1 的表达。在功能实验中，研究了 HMGB1 敲低对卵巢癌细胞生物学行为的影响。
与低侵袭性亚克隆相比，HMGB1 在高侵袭性亚克隆中过度表达。高 HMGB1 表达与不良临床病理特征相关。HMGB1 表达的敲低显著抑制卵巢癌细胞的增殖，伴随细胞周期蛋白 D1 和 PCNA 表达的降低，并抑制细胞迁移和侵袭，伴随 MMP2 和 MMP9 活性的降低。
HMGB1 是一种在卵巢癌中过度表达的新鉴定基因，与不良临床病理特征相关。HMGB1 可能成为未来卵巢癌的新生物标志物和治疗靶点。
