Fu Fan, Hu Hua, Yang Shuai, Liang Xiaoqiu
Cancer Research Institute, Key Laboratory of Tumor Cellular and Molecular Pathology, College of Hunan, University of South China, Hengyang, Hunan 421001, P.R. China.
Department of Pathology, The Fourth Hospital of Changsha, Changsha, Hunan 410006, P.R. China.
Oncol Lett. 2018 Apr;15(4):5161-5166. doi: 10.3892/ol.2018.7927. Epub 2018 Feb 2.
TERF1-interacting nuclear factor 2 (TIN2) is a key member of the protein complexes that protect telomeres. TIN2 contributes an important role in biological processes. In a previous study by the present authors, an association was reported between high TIN2 protein expression and gastric cancer. Therefore, it was hypothesized that abnormal TIN2 expression may cause the development of malignancies, including, gastric carcinomas. To investigate this hypothesis, the present study employed peptide nucleic acid fluorescence hybridization technology to analyze the human gastric epithelial GES-1 cells with high TIN2 expression or inhibited TIN2 expression. The results indicated that GES-1 cell lines with high TIN2 expression exhibited greater telomere dysfunction-induced damage compared with GES-1 cell lines with inhibited TIN2 expression. Chromosome analysis indicated that GES-1 cells with high TIN2 expression exhibited 2.48±1.30 aberrant chromosomal changes per 100 cells, that may contribute to telomere DNA damage. Therefore, aberrant chromosomal alterations may provide a novel perspective for the pathogenesis of gastric cancer.
端粒重复结合因子1相互作用核因子2（TIN2）是保护端粒的蛋白质复合物的关键成员。TIN2在生物过程中发挥着重要作用。在本作者之前的一项研究中，报道了TIN2蛋白高表达与胃癌之间的关联。因此，推测TIN2表达异常可能导致包括胃癌在内的恶性肿瘤的发生。为了验证这一假设，本研究采用肽核酸荧光杂交技术分析TIN2高表达或TIN2表达受抑制的人胃上皮GES-1细胞。结果表明，与TIN2表达受抑制的GES-1细胞系相比，TIN2高表达的GES-1细胞系表现出更大的端粒功能障碍诱导损伤。染色体分析表明，TIN2高表达的GES-1细胞每100个细胞表现出2.48±1.30个异常染色体变化，这可能导致端粒DNA损伤。因此，异常染色体改变可能为胃癌的发病机制提供一个新的视角。
