Yamada Mikako, Tsuji Naoki, Nakamura Masashi, Moriai Ryosuke, Kobayashi Daisuke, Yagihashi Atsuhito, Watanabe Naoki
Department of Laboratory Diagnosis, and Clinical Laboratory Medicine, Sapporo Medical University, School of Medicine, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan.
Anticancer Res. 2002 Nov-Dec;22(6A):3303-7.
BACKGROUND: The maintenance of telomeres may be required for long-term proliferation of tumors. Activity of telomerase, a ribonucleoprotein complex that elongates telomeres, has been found in almost all human tumors but not in adjacent normal cells. Several factors which regulate telomere length, TRF1 and 2, TIN2, tankyrase and Rap1, have been identified. TRF1, TRF2 and TIN2 are negative regulators of telomere length, while tankyrase and Rap1 act as positive regulators. In this study, we quantitated the mRNA of these five genes in gastric cancers to clarify the mechanism by which cancer cells maintain telomere length. MATERIALS AND METHODS: The expression of these five genes transcription was determined using a quantitative RT-PCR. RESULTS: TRF1, TRF2 and TIN2 mRNAs were significantly down-regulated in cancers compared to non-cancerous mucosa. Neither tankyrase nor Rap1 was upregulated in cancers. CONCLUSION: Down-regulation of TRF1, TRF2 and TIN2 gene expression may be important to maintain telomeres in gastric cancer.
背景：肿瘤的长期增殖可能需要端粒的维持。端粒酶是一种可延长端粒的核糖核蛋白复合体，几乎在所有人类肿瘤中均有活性，但在相邻的正常细胞中却未发现。已鉴定出几种调节端粒长度的因子，即TRF1和TRF2、TIN2、端锚聚合酶和Rap1。TRF1、TRF2和TIN2是端粒长度的负调节因子，而端锚聚合酶和Rap1则作为正调节因子。在本研究中，我们对胃癌中这五个基因的mRNA进行定量分析，以阐明癌细胞维持端粒长度的机制。 材料与方法：采用定量逆转录聚合酶链反应（RT-PCR）检测这五个基因转录的表达情况。 结果：与非癌黏膜相比，癌症中TRF1、TRF2和TIN2的mRNA显著下调。癌症中端锚聚合酶和Rap1均未上调。 结论：TRF1、TRF2和TIN2基因表达的下调可能对维持胃癌中的端粒很重要。
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