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在体外3' 端形成过程中，哺乳动物组蛋白前体mRNA上的两个保守信号均与小核核糖核蛋白相关联。

Both conserved signals on mammalian histone pre-mRNAs associate with small nuclear ribonucleoproteins during 3' end formation in vitro.

作者信息

Mowry K L, Steitz J A

出版信息

Mol Cell Biol. 1987 May;7(5):1663-72. doi: 10.1128/mcb.7.5.1663-1672.1987.

DOI:10.1128/mcb.7.5.1663-1672.1987
PMID:2955216
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365266/
Abstract

Pre-mRNA substrates containing sequences from human and mouse histone genes are accurately processed in a HeLa cell nuclear extract to generate mature 3' termini. When in vitro processing reactions containing either human histone H3 or mouse histone H3 transcripts are treated with RNase T1 and probed with antibodies specific for the Sm protein determinants or for the trimethylguanosine cap structure unique to the U RNAs present in small nuclear ribonucleoproteins, RNA fragments that encompass the site of 3' end formation on the pre-mRNA transcript are selectively recovered. Several different interactions are detected: at time zero, the protected region contains the upstream conserved hairpin loop structure; at later times during the reaction, protection extends beyond the site of 3' end formation to include the downstream conserved sequence element and the 5' cap of the transcript is bound as well. Possible interactions between Sm small nuclear ribonucleoproteins and these conserved sequence elements in histone pre-mRNAs are discussed.

摘要

含有来自人类和小鼠组蛋白基因序列的前体mRNA底物在HeLa细胞核提取物中被精确加工，以产生成熟的3'末端。当用RNase T1处理含有人类组蛋白H3或小鼠组蛋白H3转录本的体外加工反应，并使用针对Sm蛋白决定簇或小核核糖核蛋白中存在的U RNA特有的三甲基鸟苷帽结构的抗体进行探测时，包含前体mRNA转录本上3'末端形成位点的RNA片段被选择性回收。检测到几种不同的相互作用：在反应开始时，受保护区域包含上游保守的发夹环结构；在反应后期，保护范围延伸到3'末端形成位点之外，包括下游保守序列元件，并且转录本的5'帽也被结合。本文讨论了Sm小核核糖核蛋白与组蛋白前体mRNA中这些保守序列元件之间可能的相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/169969a7cf98/molcellb00077-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/7a17ca12167a/molcellb00077-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/702e83990d78/molcellb00077-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/e64929c8925a/molcellb00077-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/fc42d68fc3ff/molcellb00077-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/169969a7cf98/molcellb00077-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/7a17ca12167a/molcellb00077-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/702e83990d78/molcellb00077-0093-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/e64929c8925a/molcellb00077-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/fc42d68fc3ff/molcellb00077-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9de/365266/169969a7cf98/molcellb00077-0097-a.jpg

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Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease.
果蝇U7小核核糖核蛋白（snRNP）的Lsm10和Lsm11蛋白是组蛋白前体mRNA加工所必需的，并且在发育过程中发挥着重要作用。
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