获取急性脑切片。
Obtaining Acute Brain Slices.
作者信息
Papouin Thomas, Haydon Philip G
机构信息
Department of Neuroscience, Tufts University School of Medicine, Boston, MA, USA.
出版信息
Bio Protoc. 2018 Jan 20;8(2). doi: 10.21769/BioProtoc.2699.
Abstract
Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the dissection, extraction and acute slicing of the mouse brain, including tips and list of material required.
摘要
获取用于电生理学或安培测量记录的急性脑片，在神经科学领域的大多数实验室中已成为常规程序。然而，描述逐步过程的方案却很少，特别是对于常规急性制备，如从小鼠海马体获取脑片。在此，我们提供了一份关于小鼠脑解剖、提取和急性切片的详细方案，包括技巧和所需材料清单。
相似文献
1
Obtaining Acute Brain Slices.
Bio Protoc. 2018 Jan 20;8(2). doi: 10.21769/BioProtoc.2699.
2
Horizontal Hippocampal Slices of the Mouse Brain.
J Vis Exp. 2020 Sep 22(163). doi: 10.3791/61753.
3
Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples.
Bio Protoc. 2020 Oct 5;10(19):e3771. doi: 10.21769/BioProtoc.3771.
4
Acute Mouse Brain Slicing to Investigate Spontaneous Hippocampal Network Activity.
J Vis Exp. 2020 Aug 28(162). doi: 10.3791/61704.
5
Antiseizure drugs differentially modulate θ-burst induced long-term potentiation in C57BL/6 mice.
Epilepsia. 2014 Feb;55(2):214-23. doi: 10.1111/epi.12524. Epub 2014 Jan 21.
6
Preparation of hippocampal brain slices.
Curr Protoc Neurosci. 2001 May;Chapter 6:Unit 6.4. doi: 10.1002/0471142301.ns0604s00.
7
Effects of variations in hippocampal slice preparation protocol on the electrophysiological stability, epileptogenicity and graded hypoxia responses of CA1 neurons.
Brain Res. 1997 Nov 14;775(1-2):134-43. doi: 10.1016/s0006-8993(97)00893-7.
8
Single or Double Patch-Clamp Recordings In Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and Optogenetics.
Methods Mol Biol. 2021;2188:285-309. doi: 10.1007/978-1-0716-0818-0_15.
9
Slice it hot: acute adult brain slicing in physiological temperature.
J Vis Exp. 2014 Oct 30(92):e52068. doi: 10.3791/52068.
10
Design and application of a novel brain slice system that permits independent electrophysiological recordings from multiple slices.
J Neurosci Methods. 2004 Jan 30;132(2):137-48. doi: 10.1016/j.jneumeth.2003.08.015.
引用本文的文献
1
Multi-photon, label-free photoacoustic and optical imaging of NADH in brain cells.
Light Sci Appl. 2025 Aug 7;14(1):264. doi: 10.1038/s41377-025-01895-x.
2
A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue.
Cell Rep Methods. 2025 Jul 21;5(7):101080. doi: 10.1016/j.crmeth.2025.101080. Epub 2025 Jun 16.
3
Norepinephrine signals through astrocytes to modulate synapses.
Science. 2025 May 15;388(6748):776-783. doi: 10.1126/science.adq5480.
4
Molecular Engineering of Virus Tropism.
Int J Mol Sci. 2024 Oct 15;25(20):11094. doi: 10.3390/ijms252011094.
5
Norepinephrine Signals Through Astrocytes To Modulate Synapses.
bioRxiv. 2024 May 22:2024.05.21.595135. doi: 10.1101/2024.05.21.595135.
6
MeCP2 represses the activity of topoisomerase IIβ in long neuronal genes.
Cell Rep. 2023 Dec 26;42(12):113538. doi: 10.1016/j.celrep.2023.113538. Epub 2023 Dec 12.
7
Acute brain slice elastic modulus decreases over time.
Sci Rep. 2023 Aug 7;13(1):12826. doi: 10.1038/s41598-023-40074-z.
8
Evaluation of prophylactic efficacy of sodium thiosulfate in combating I/R injury in rat brain: exploring its efficiency further in vascular calcified brain slice model.
Naunyn Schmiedebergs Arch Pharmacol. 2023 Oct;396(10):2587-2598. doi: 10.1007/s00210-023-02481-x. Epub 2023 Apr 14.
9
A single-cell RNA labeling strategy for measuring stress response upon tissue dissociation.
Mol Syst Biol. 2023 Feb 10;19(2):e11147. doi: 10.15252/msb.202211147. Epub 2022 Dec 27.
10
Estrous Cycle Mediates Midbrain Neuron Excitability Altering Social Behavior upon Stress.
J Neurosci. 2023 Feb 1;43(5):736-748. doi: 10.1523/JNEUROSCI.1504-22.2022. Epub 2022 Dec 22.
本文引用的文献
1
D-serine Measurements in Brain Slices or Other Tissue Explants.
Bio Protoc. 2018 Jan 20;8(2). doi: 10.21769/BioProtoc.2698.
2
An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation .
Bio Protoc. 2017 Jan 20;7(2). doi: 10.21769/BioProtoc.2102.
3
Septal Cholinergic Neuromodulation Tunes the Astrocyte-Dependent Gating of Hippocampal NMDA Receptors to Wakefulness.
Neuron. 2017 May 17;94(4):840-854.e7. doi: 10.1016/j.neuron.2017.04.021. Epub 2017 May 4.
Zotero 插件