Bernstein Center for Computational Neuroscience, Humboldt-Universität zu Berlin, Berlin, Germany.
CNRS (Integrative Neuroscience and Cognition Center, UMR 8002), Paris, France.
Methods Mol Biol. 2021;2188:285-309. doi: 10.1007/978-1-0716-0818-0_15.
Patch-clamp recordings are the method of choice to define cell-type specific electrophysiological properties of single neurons and the synaptic connectivity between pairs of connected neurons in brain slices. In combination with optogenetic tools, patch-clamp recordings allow for the investigation of long-range afferent connectivity from identified distant brain areas. Here we describe the necessary equipment to carry out patch clamp recordings, surgical methods for dissection and preparation of horizontal brain slices containing the hippocampus, and a step-by-step guide for establishing patch clamp recordings in the whole-cell configuration. We provide protocols for single neuron stimulation via the patch pipette and for photostimulation experiments that activate axon terminals expressing light sensitive ion channels.
膜片钳记录是一种选择，用于定义单个神经元的细胞类型特异性电生理特性，以及脑片内成对连接神经元之间的突触连接。与光遗传学工具结合使用，膜片钳记录允许研究从已识别的远距离脑区传入的长程传入连接。本文描述了进行膜片钳记录所需的设备，用于解剖和制备包含海马体的水平脑片的手术方法，以及在全细胞构型中建立膜片钳记录的分步指南。我们提供了通过膜片钳对单个神经元进行刺激的方案，以及用于激活表达光敏感离子通道的轴突末梢的光刺激实验的方案。
