Emerg Infect Dis. 2018 Apr;24(4):673-682. doi: 10.3201/eid2404.171480.
In September 2015, 4CMenB meningococcal vaccine was introduced into the United Kingdom infant immunization program without phase 3 trial information. Understanding the effect of this program requires enhanced surveillance of invasive meningococcal disease (IMD) Neisseria meningitidis isolates and comparison with prevaccination isolates. Bexsero Antigen Sequence Types (BASTs) were used to analyze whole-genome sequences of 3,073 prevaccine IMD N. meningitidis isolates obtained during 2010-2016. Isolates exhibited 803 BASTs among 31 clonal complexes. Frequencies of antigen peptide variants were factor H binding protein 1, 13.4%; Neisserial heparin-binding antigen 2, 13.8%; Neisseria adhesin A 8, 0.8%; and Porin A-VR2:P1.4,10.9%. In 2015-16, serogroup B isolates showed the highest proportion (35.7%) of exact matches to >1 Bexsero components. Serogroup W isolates showed the highest proportion (93.9%) of putatively cross-reactive variants of Bexsero antigens. Results highlighted the likely role of cross-reactive antigens. BAST surveillance of meningococcal whole-genome sequence data is rapid, scalable, and portable and enables international comparisons of isolates.
2015 年 9 月，4CMenB 脑膜炎球菌疫苗在没有 3 期临床试验信息的情况下被引入英国婴儿免疫计划。了解该计划的效果需要加强对侵袭性脑膜炎球菌病（IMD）奈瑟氏脑膜炎球菌分离株的监测，并与疫苗接种前的分离株进行比较。Bexsero 抗原序列类型（BAST）用于分析 2010-2016 年期间获得的 3073 例疫苗前 IMD N. meningitidis 分离株的全基因组序列。分离株在 31 个克隆复合物中表现出 803 个 BAST。抗原肽变体的频率为因子 H 结合蛋白 1，占 13.4%；奈瑟氏肝素结合抗原 2，占 13.8%；Neisseria 黏附素 A8，占 0.8%；Porin A-VR2：P1.4，10.9%。2015-16 年，B 群分离株与>1 种 Bexsero 成分完全匹配的比例最高（35.7%）。W 群分离株 Bexsero 抗原的交叉反应变体比例最高（93.9%）。结果突出了交叉反应抗原的可能作用。脑膜炎球菌全基因组序列数据的 BAST 监测快速、可扩展且便携，能够对分离株进行国际比较。
