Centre of Bacteriology, Institute Adolfo Lutz (IAL), São Paulo, Brazil.
Strategic Laboratory, Institute Adolfo Lutz (IAL), São Paulo, Brazil.
PLoS One. 2020 Dec 21;15(12):e0243375. doi: 10.1371/journal.pone.0243375. eCollection 2020.
Neisseria meningitidis serogroup B remains a prominent cause of invasive meningococcal disease (IMD) in Brazil. Because two novel protein-based vaccines against serogroup B are available, the main purpose of this study was to provide data on the diversity and distribution of meningococcal vaccine antigen types circulating in Brazil.
Genetic lineages, vaccine antigen types, and allele types of antimicrobial-associated resistance genes based on whole-genome sequencing of a collection of 145 Neisseria meningitidis serogroup B invasive strains recovered in Brazil from 2016 to 2018 were collected.
A total of 11 clonal complexes (ccs) were identified among the 145 isolates, four of which were predominant, namely, cc461, cc35, cc32, and cc213, accounting for 72.0% of isolates. The most prevalent fHbp peptides were 24 (subfamily A/variant 2), 47 (subfamily A/variant 3), 1 (subfamily B/variant 1) and 45 (subfamily A/variant 3), which were predominantly associated with cc35, cc461, cc32, and cc213, respectively. The NadA peptide was detected in only 26.2% of the isolates. The most frequent NadA peptide 1 was found almost exclusively in cc32. We found seven NHBA peptides that accounted for 74.5% of isolates, and the newly described peptide 1390 was the most prevalent peptide exclusively associated with cc461. Mutated penA alleles were detected in 56.5% of the isolates, whereas no rpoB and gyrA mutant alleles were found.
During the study period, changes in the clonal structure of circulating strains were observed, without a predominance of a single hyperinvasive lineage, indicating that an epidemiologic shift has occurred that led to a diversity of vaccine antigen types in recent years in Brazil.
脑膜炎奈瑟菌 B 群仍然是巴西侵袭性脑膜炎球菌病（IMD）的主要原因。由于有两种针对 B 群的新型蛋白疫苗，因此本研究的主要目的是提供巴西流行的脑膜炎球菌疫苗抗原类型的多样性和分布数据。
收集了 2016 年至 2018 年期间在巴西分离的 145 株 B 群侵袭性脑膜炎奈瑟氏菌的全基因组序列，基于全基因组测序，对其遗传谱系、疫苗抗原类型和抗菌相关耐药基因的等位基因类型进行了分析。
在 145 株分离株中鉴定出 11 个克隆复合体（cc），其中 4 个为主要流行株，分别为 cc461、cc35、cc32 和 cc213，占分离株的 72.0%。最常见的 fHbp 肽是 24（亚家族 A/变体 2）、47（亚家族 A/变体 3）、1（亚家族 B/变体 1）和 45（亚家族 A/变体 3），分别主要与 cc35、cc461、cc32 和 cc213 相关。仅在 26.2%的分离株中检测到 NadA 肽。最常见的 NadA 肽 1 几乎仅存在于 cc32 中。我们发现了 7 种 NHBA 肽，占分离株的 74.5%，新描述的肽 1390 是唯一与 cc461 相关的最常见肽。在 56.5%的分离株中检测到突变的 penA 等位基因，而未发现 rpoB 和 gyrA 突变等位基因。
在研究期间，观察到流行菌株的克隆结构发生了变化，没有单一的超侵袭谱系占主导地位，这表明近年来巴西发生了流行病学转变，导致疫苗抗原类型多样化。
